Design and characterization of antibiotic-free lyotropic liquid crystalline coatings based on binary docosahexaenoic acid monoglyceride/glycerol monooleate systems for combating orthopedic implant-associated infections

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dc.contributor.authorAkay, Seref
dc.contributor.authorCiofu, Oana
dc.contributor.authorGow, Jonathan Michael
dc.contributor.authorPosselt, Dorthe
dc.contributor.authorMarmiroli, Benedetta
dc.contributor.authorSartori, Barbara
dc.contributor.authorYaghmur, Anan
dc.date.accessioned2026-01-24T12:31:12Z
dc.date.available2026-01-24T12:31:12Z
dc.date.issued2026
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractThere is a growing interest in innovative strategies for effectively preventing implant-associated bacterial infections. Here, we report on a simple-by-design approach for production of antibacterial coatings from docosahexaenoic acid monoacylglycerol (MAG-DHA) and glycerol monooleate (GMO). In addition to its amphiphilic nature, MAG-DHA is a safe precursor of docosahexaenoic acid (DHA) and has different beneficial health effects, including antibacterial activities. Using binary combinations of MAG-DHA and GMO, we describe the structural features and the antibacterial activities of antibiotic-free non-lamellar liquid crystalline (LLC) coatings. Small-angle X-ray scattering (SAXS) and in situ grazing incidence small-angle X-ray scattering (GISAXS) were employed to gain insight into the structural features of the fully hydrated coatings and the hydration-induced dynamic self-assembly of MAG-DHA and GMO on model implants. In a lipid composition-dependent manner, SAXS results revealed hydration-induced formation of different inverse LLC self-assemblies, including hexagonal (H2) and bicontinuous cubic (Q2) phases. Further, GISAXS analysis showed that lipid composition and employed relative humidity level play important roles in controlling the out-of-equilibrium self-assembly properties. Moreover, the coatings containing MAG-DHA displayed unique inherent antibacterial activities against Staphylococcus aureus and Staphylococcus epidermidis strains. This study describes the first antibiotic-free coatings with nanostructural architectures and inherent antibacterial activities for orthopedic implants.
dc.description.sponsorshipEuropean Union's Horizon Europe under the Marie Sklodowska-Curie Postdoctoral Fellowships programme [101107704]; Novo Nordisk Foundation [NNF21OC0068491]; Danish Natural Sciences Research Council (DanScatt) for SAXS experiments and the Danish Council for Independent Research | Technology and Production Sciences [DFF-3105 00039B]; CERIC-ERIC Consortium
dc.description.sponsorshipThe study funded by the European Union's Horizon Europe under the Marie Sklodowska-Curie Postdoctoral Fellowships programme (Grant No: 101107704). The Novo Nordisk Foundation is gratefully acknowledged for funding RUCSAXS-Roskilde University Interdisciplinary Xray Scattering Hub-with grant No: NNF21OC0068491. A.Y. further acknowledges financial support by the Danish Natural Sciences Research Council (DanScatt) for SAXS experiments and the Danish Council for Independent Research | Technology and Production Sciences (reference DFF-3105 00039B). S.A. acknowledge the CERIC-ERIC Consortium for access to the Austrian SAXS beamline at the Elettra Synchrotron (ELETTRA, Trieste, Italy).
dc.identifier.doi10.1016/j.jcis.2025.139493
dc.identifier.issn0021-9797
dc.identifier.issn1095-7103
dc.identifier.pmid41274159
dc.identifier.scopus2-s2.0-105022110445
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jcis.2025.139493
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5722
dc.identifier.volume705
dc.identifier.wosWOS:001628221700007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofJournal of Colloid and Interface Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectAntibacterial coatings
dc.subjectBiofilms
dc.subjectDocosahexaenoic acid monoglyceride
dc.subjectGISAXS
dc.subjectInverse bicontinuous cubic phase
dc.subjectInverse hexagonal phase
dc.subjectOrthopedic implants
dc.titleDesign and characterization of antibiotic-free lyotropic liquid crystalline coatings based on binary docosahexaenoic acid monoglyceride/glycerol monooleate systems for combating orthopedic implant-associated infections
dc.typeArticle

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