Design and characterization of antibiotic-free lyotropic liquid crystalline coatings based on binary docosahexaenoic acid monoglyceride/glycerol monooleate systems for combating orthopedic implant-associated infections
[ X ]
Tarih
2026
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
There is a growing interest in innovative strategies for effectively preventing implant-associated bacterial infections. Here, we report on a simple-by-design approach for production of antibacterial coatings from docosahexaenoic acid monoacylglycerol (MAG-DHA) and glycerol monooleate (GMO). In addition to its amphiphilic nature, MAG-DHA is a safe precursor of docosahexaenoic acid (DHA) and has different beneficial health effects, including antibacterial activities. Using binary combinations of MAG-DHA and GMO, we describe the structural features and the antibacterial activities of antibiotic-free non-lamellar liquid crystalline (LLC) coatings. Small-angle X-ray scattering (SAXS) and in situ grazing incidence small-angle X-ray scattering (GISAXS) were employed to gain insight into the structural features of the fully hydrated coatings and the hydration-induced dynamic self-assembly of MAG-DHA and GMO on model implants. In a lipid composition-dependent manner, SAXS results revealed hydration-induced formation of different inverse LLC self-assemblies, including hexagonal (H2) and bicontinuous cubic (Q2) phases. Further, GISAXS analysis showed that lipid composition and employed relative humidity level play important roles in controlling the out-of-equilibrium self-assembly properties. Moreover, the coatings containing MAG-DHA displayed unique inherent antibacterial activities against Staphylococcus aureus and Staphylococcus epidermidis strains. This study describes the first antibiotic-free coatings with nanostructural architectures and inherent antibacterial activities for orthopedic implants.
Açıklama
Anahtar Kelimeler
Antibacterial coatings, Biofilms, Docosahexaenoic acid monoglyceride, GISAXS, Inverse bicontinuous cubic phase, Inverse hexagonal phase, Orthopedic implants
Kaynak
Journal of Colloid and Interface Science
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
705
