Sapienic Acid Metabolism Influences Membrane Plasticity and Protein Signaling in Breast Cancer Cell Lines
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract: The importance of sapienic acid (6c-16:1), a monounsaturated fatty acid of the n-10 family formed from palmitic acid by delta-6 desaturase, and of its metabolism to 8c-18:1 and sebaleic acid (5c,8c-18:2) has been recently assessed in cancer. Data are lacking on the association between signaling cascades and exposure to sapienic acid comparing cell lines of the same cancer type. We used 50 µM sapienic acid supplementation, a non-toxic concentration, to cultivate MCF-7 and 2 triplenegative breast cancer cells (TNBC), MDA-MB-231 and BT-20. We followed up for three hours regarding membrane fatty acid remodeling by fatty acid-based membrane lipidome analysis and expression/phosphorylation of EGFR (epithelial growth factor receptor), mTOR (mammalian target of rapamycin) and AKT (protein kinase B) by Western blotting as an oncogenic signaling cascade. Results evidenced consistent differences among the three cell lines in the metabolism of n-10 fatty acids and signaling. Here, a new scenario is proposed for the role of sapienic acid: one based on changes in membrane composition and properties, and the other based on changes in expression/activation of growth factors and signaling cascades. This knowledge can indicate additional players and synergies in breast cancer cell metabolism, inspiring translational applications of tailored membrane lipid strategies to assist pharmacological interventions.
Açıklama
Anahtar Kelimeler
Membrane fatty acids, Breast cancer, Membrane remodeling, Fatty acid supplementation, Cancer signaling activation, Membrane lipidome
Kaynak
Cells
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
11
Sayı
2
Künye
Bağlantı
https://www.scopus.com/record/display.uri?eid=2-s2.0-85122674599&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=20c4fe371b9b908d4f7e419f791331ff&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=72&s=AF-ID%28%22Alanya+Alaaddin+Keykubat+University%22+60198720%29+AND+SUBJAREA%28MEDI%29&relpos=87&citeCnt=9&searchTerm=
https://hdl.handle.net/20.500.12868/2412
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773705/pdf/cells-11-00225.pdf
https://hdl.handle.net/20.500.12868/2412
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773705/pdf/cells-11-00225.pdf