Sapienic Acid Metabolism Influences Membrane Plasticity and Protein Signaling in Breast Cancer Cell Lines

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dc.contributor.authorKüçüksayan, Ertan
dc.contributor.authorSansone, Anna
dc.contributor.authorChatgilialoglu, Chryssostomos
dc.contributor.authorÖzben, Tomris
dc.contributor.authorTekeli, Demet
dc.contributor.authorTalibova, Günel
dc.contributor.authorFerreri, Carla
dc.date.accessioned2023-10-11T08:06:41Z
dc.date.available2023-10-11T08:06:41Z
dc.date.issued2022
dc.departmentALKÜ, Fakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractAbstract: The importance of sapienic acid (6c-16:1), a monounsaturated fatty acid of the n-10 family formed from palmitic acid by delta-6 desaturase, and of its metabolism to 8c-18:1 and sebaleic acid (5c,8c-18:2) has been recently assessed in cancer. Data are lacking on the association between signaling cascades and exposure to sapienic acid comparing cell lines of the same cancer type. We used 50 µM sapienic acid supplementation, a non-toxic concentration, to cultivate MCF-7 and 2 triplenegative breast cancer cells (TNBC), MDA-MB-231 and BT-20. We followed up for three hours regarding membrane fatty acid remodeling by fatty acid-based membrane lipidome analysis and expression/phosphorylation of EGFR (epithelial growth factor receptor), mTOR (mammalian target of rapamycin) and AKT (protein kinase B) by Western blotting as an oncogenic signaling cascade. Results evidenced consistent differences among the three cell lines in the metabolism of n-10 fatty acids and signaling. Here, a new scenario is proposed for the role of sapienic acid: one based on changes in membrane composition and properties, and the other based on changes in expression/activation of growth factors and signaling cascades. This knowledge can indicate additional players and synergies in breast cancer cell metabolism, inspiring translational applications of tailored membrane lipid strategies to assist pharmacological interventions.
dc.identifier.doi10.3390/cells11020225
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85122674599&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=20c4fe371b9b908d4f7e419f791331ff&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=72&s=AF-ID%28%22Alanya+Alaaddin+Keykubat+University%22+60198720%29+AND+SUBJAREA%28MEDI%29&relpos=87&citeCnt=9&searchTerm=
dc.identifier.urihttps://hdl.handle.net/20.500.12868/2412
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773705/pdf/cells-11-00225.pdf
dc.identifier.volume11en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.relation.ispartofCells
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMembrane fatty acids
dc.subjectBreast cancer
dc.subjectMembrane remodeling
dc.subjectFatty acid supplementation
dc.subjectCancer signaling activation
dc.subjectMembrane lipidome
dc.titleSapienic Acid Metabolism Influences Membrane Plasticity and Protein Signaling in Breast Cancer Cell Lines
dc.typeArticle

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