Tricine-modified chitosan as a strategy for enhancing hydrophilicity and gene delivery

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dc.authorid0000-0002-9127-2380
dc.authorid0000-0002-3052-4556
dc.authorid0000-0003-2185-2619
dc.contributor.authorTantan, Yasemin
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorBala, Kevser
dc.contributor.authorSenturk, Sema
dc.contributor.authorFidan, Emine Buera Eker
dc.contributor.authorCelik, Sibel
dc.contributor.authorDemir, Kamber
dc.date.accessioned2026-01-24T12:31:07Z
dc.date.available2026-01-24T12:31:07Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIn this study, we investigated the effect of chitosan modification with tricine on transfection efficiency by preserving its ability to form complexes with plasmid DNA (pDNA) and increasing its hydrophilicity. The inherent limitations of chitosan, such as poor solubility at physiological pH, insufficient cellular uptake, and strong ionic interactions with pDNA, typically result in low transfection efficiency. To overcome these challenges, Tricine, a hydrophilic molecule containing a secondary amine group, was conjugated to chitosan. Chitosan of three different molecular weights (low, medium, and high) was modified with tricine. Structural characterization of the modified chitosan was conducted using Fourier Transformed Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR) analyses. The effects of tricine modification were assessed in terms of hydrophilicity/hydrophobicity, proton buffering capacity, particle size, PDI and zeta potential. Tricine modified low molecular weight chitosan nanoparticles (nLMWChiTri), which exhibit suitable properties for gene transfer studies, were evaluated regarding pDNA complexation ability, cytotoxicity and in vitro transfection efficiency. The results demonstrated that tricine modification enhanced the gene transfer potential of chitosan, making it competitive with the commercial transfection agent LipofectamineTM 2000 and offering a promising strategy for non-viral gene therapy applications. Furthermore, the biocompatibility and biodegradability of chitosan, combined with the improved hydrophilicity provided by tricine, makes nLMWChiTri a safer and more sustainable option for repeated use in gene delivery, overcoming the major limitations associated with other synthetic vectors such as LipofectamineTM 2000.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye [1919B012107832/2209-A]; Presidency of the Republic of Turkey, Strategy and Budget Office [DPT-2019K12-149071]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkiye (Project Number: 1919B012107832/2209-A) and by the project DPT-2019K12-149071 funded by the Presidency of the Republic of Turkey, Strategy and Budget Office.
dc.identifier.doi10.1016/j.carres.2024.109326
dc.identifier.issn0008-6215
dc.identifier.issn1873-426X
dc.identifier.pmid39579713
dc.identifier.scopus2-s2.0-85209679298
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.carres.2024.109326
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5657
dc.identifier.volume547
dc.identifier.wosWOS:001364404100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofCarbohydrate Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectNon-viral vectors
dc.subjectChitosan modification
dc.subjectGene delivery
dc.subjectTricine
dc.subjectHydrophilicity
dc.titleTricine-modified chitosan as a strategy for enhancing hydrophilicity and gene delivery
dc.typeArticle

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