Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

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dc.authorid0000-0002-7248-7933
dc.authorid0000-0003-2594-502X
dc.authorid0000-0001-8379-5678
dc.authorid0000-0002-0726-803X
dc.contributor.authorGoksu, Azize Yasemin
dc.contributor.authorKocanci, Fatma Gonca
dc.contributor.authorAkinci, Ersin
dc.contributor.authorDemir-Dora, Devrim
dc.contributor.authorErendor, Fulya
dc.contributor.authorSanlioglu, Salih
dc.contributor.authorUysal, Hilmi
dc.date.accessioned2026-01-24T12:29:02Z
dc.date.available2026-01-24T12:29:02Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractA common pathological hallmark of neurodegenerative disorders is neuronal cell death, accompanied by neuroinflammation and oxidative stress. The vasoactive intestinal peptide (VIP) is a pleiotropic peptide that combines neuroprotective and immunomodulatory actions. The gene therapy field shows long-term promise for treating a wide range of neurodegenerative diseases (ND). In this study, we aimed to investigate the in vitro efficacy of transduction of microglia using lentiviral gene therapy vectors encoding VIP (LentiVIP). Additionally, we tested the protective effects of the secretome derived from LentiVIP-infected immortalized human microglia HMC3 cells, and cells treated with Synthetic VIP (SynVIP), against toxin-induced neurodegeneration. First, LentiVIP, which stably expresses VIP, was generated and purified. VIP secretion in microglial conditioned media (MG CM) for LentiVIP-infected HMC3 microglia cells was confirmed. Microglia cells were activated with lipopolysaccharide, and groups were formed as follows: 1) Control, 2) SynVIP-treated, or 3) LentiVIP-transduced. These MG CM were applied on an in vitro neurodegenerative model formed by differentiated (d)-SH-SY5Y cells. Then, cell survival analysis and apoptotic nuclear staining, besides measurement of oxidative/inflammatory parameters in CM of cells were performed. Activated MG CM reduced survival rates of both control and toxin-applied (d)-SH-SY5Y cells, whereas LentiVIP-infected MG CM and SynVIP-treated ones exhibited better survival rates. These findings were supported by apoptotic nuclear evaluations of (d)-SH-SY5Y cells, alongside oxidative/inflammatory parameters in their CM. LentiVIP seems worthy of further studies for the treatment of ND because of the potential of gene therapy to treat diseases effectively with a single injection. Cultured microglial cells were activated with LPS, and then grouped as: 1) Control, 2) SynVIP-treated, or 3) LentiVIP-transduced microglia. Subsequently, conditioned media of microglial groups were applied on differentiated-SH-SY5Y cells that were exposed to MPTP (a neurotoxin) or not. Several analysis and tests were performed to investigate the protective effects of SynVIP or LentiVIP against toxicity of MPTP or microglial secretome. image
dc.description.sponsorshipScientific Research Council of Akdeniz University; [TKA-2018-3933]
dc.description.sponsorshipThis study was supported by the Scientific Research Council of Akdeniz University (Grant number AU, TKA-2018-3933). The authors express their sincere thanks to Prof. Dr. Nuray Erin, Faculty of Medicine, Akdeniz University, for her support in ELISA studies of the project.
dc.identifier.doi10.1111/ejn.16273
dc.identifier.endpage2015
dc.identifier.issn0953-816X
dc.identifier.issn1460-9568
dc.identifier.issue8
dc.identifier.pmid38382910
dc.identifier.scopus2-s2.0-85186434654
dc.identifier.scopusqualityQ2
dc.identifier.startpage1993
dc.identifier.urihttps://doi.org/10.1111/ejn.16273
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5085
dc.identifier.volume59
dc.identifier.wosWOS:001169293600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofEuropean Journal of Neuroscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectgene therapy
dc.subjectLentiVIP
dc.subjectmicroglia
dc.subjectneurodegeneration
dc.subjectvasoactive intestinal peptide
dc.titleMicroglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration
dc.typeArticle

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