An in vitro pilot study investigating placenta-derived mesenchymal stem cell coating on polypropylene mesh materials

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dc.authorid0000-0001-5284-7439
dc.authorid0000-0002-4940-8862
dc.authorid0000-0002-9573-5688
dc.contributor.authorAslan, Erdogan
dc.contributor.authorMaytalman, Erkan
dc.contributor.authorSamur, Dilara Nemutlu
dc.contributor.authorKole, Emre
dc.contributor.authorGunizi, Ozlem Ceren
dc.date.accessioned2026-01-24T12:30:52Z
dc.date.available2026-01-24T12:30:52Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIntroduction and hypothesis: Polypropylene meshes (PM) used in pelvic organ prolapse surgery are being withdrawn from the market. Although concerns about the usage of PMs in stress incontinence surgery have been raised, it is still one of the best methods of curing stress urinary incontinence. With advancements in stem cell-based therapies, especially mesenchymal stem cells (MSCs), it is believed that coating the synthetic meshes with MSCs may minimize excessive tissue reactions ultimately leading to clinical problems such as pain, erosion or extrusion of the implanted material. In our study we tried to show the possibility of coating the PM with placenta-derived MSCs.Methods: Mesenchymal stem cells obtained from six placentas were isolated, cultured, and identified. MSCs were then soaked in either fibronectin or collagen prior to co-culturing with strips of PMs. One group is used as a control, and hence was not pretreated before co-culturing. Specimens were fixed and stained with both Gram and hematoxylin and eosin and marked with Vybran Dil and DAPI. All preparations were examined under a light microscope. The IMAGEJ program was utilized to determine the surface area of meshes coated with MSCs.Results: We clearly showed that PMs can be coated successfully with placenta-derived MSCs. The percentage of the coated area is significantly increased when meshes were pretreated with fibronectin or collagen (p<0.0001).Conclusions: Placenta-derived MSCs can successfully coat PMs. The immunomodulatory properties of MSCs, which may be of great advantage in preventing the side effects of meshes, should be tested by in vivo and hopefully human studies before clinical applications.
dc.description.sponsorshipAlanya Alaaddin Keykubat University Scientific Research and Project Board [2021-04-03-MAP01]
dc.description.sponsorshipThis study was funded by Alanya Alaaddin Keykubat University Scientific Research and Project Board (2021-04-03-MAP01).
dc.identifier.doi10.1007/s00192-023-05687-y
dc.identifier.endpage559
dc.identifier.issn0937-3462
dc.identifier.issn1433-3023
dc.identifier.issue3
dc.identifier.pmid38206335
dc.identifier.scopus2-s2.0-85181934771
dc.identifier.scopusqualityQ2
dc.identifier.startpage553
dc.identifier.urihttps://doi.org/10.1007/s00192-023-05687-y
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5496
dc.identifier.volume35
dc.identifier.wosWOS:001141607900006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer London Ltd
dc.relation.ispartofInternational Urogynecology Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectPlacenta-derived mesenchymal stem cells
dc.subjectPolypropylene mesh
dc.subjectCoating
dc.titleAn in vitro pilot study investigating placenta-derived mesenchymal stem cell coating on polypropylene mesh materials
dc.typeArticle

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