Antiproliferative and apoptotic effects of Pervari honey on SH-SY5Y neuroblastoma cells

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dc.authorid0000-0002-3555-3946
dc.authorid0000-0001-6473-5813
dc.authorid0000-0003-4390-5769
dc.contributor.authorAltin-Celik, Pinar
dc.contributor.authorDerya-Andeden, Muazzez
dc.contributor.authorEciroglu-Sarban, Hamiyet
dc.contributor.authorDonmez-Altuntas, Hamiyet
dc.date.accessioned2026-01-24T12:31:00Z
dc.date.available2026-01-24T12:31:00Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractNeuroblastoma is a complex and heterogeneous pediatric malignancy. Treatment options for neuroblastoma include surgery, chemotherapy, radiotherapy, autologous stem cell transplantation, and emerging immunotherapies. However, these approaches often coincide with oxidative stress, highlighting the potential role of natural antioxidants in chemoprevention and chemotherapy. Honey has long been recognized for its medicinal properties, including antimicrobial, anti-inflammatory, and antioxidant effects. Pervari honey (PH), produced by bees that forage on a diverse array of native flora, is a distinctive honey originating from southeastern T & uuml;rkiye. This study evaluated the antiproliferative, anti-tumor, apoptotic, and inflammatory effects of PH on SH-SY5Y human neuroblastoma cells. Cell viability was measured using an MTT assay, while apoptosis was evaluated using an Annexin V-FITC/7-AAD staining test. The RT-qPCR method was used to quantify the expression of IL-1 beta, IL-6, TNF-alpha, NF-kappa B, Caspase-3, MMP-2, MMP-9, BAX, and BCL-2 genes. PH significantly reduced SH-SY5Y cell viability in a dose- and time-dependent manner (p < 0.05-p < 0.01). It induced apoptosis by upregulating BAX and Caspase-3 while downregulating BCL-2 (p < 0.05-p < 0.001). Additionally, it increased NF-kappa B expression (p < 0.05-p < 0.001) and modulated inflammatory (IL-1 beta, IL-6, and TNF-alpha) cytokines (p < 0.05-p < 0.01). MMP-2 and MMP-9 levels were significantly elevated (p < 0.05-p < 0.001), suggesting enhanced extracellular matrix remodeling. These in vitro findings indicate that PH may exert anti-tumor potential and possess immunomodulatory properties. Furthermore, in vitro and in vivo studies are needed to explore its therapeutic applicability.
dc.identifier.doi10.1007/s12032-025-02963-3
dc.identifier.issn1357-0560
dc.identifier.issn1559-131X
dc.identifier.issue9
dc.identifier.pmid40736760
dc.identifier.scopus2-s2.0-105012177115
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s12032-025-02963-3
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5579
dc.identifier.volume42
dc.identifier.wosWOS:001541369100004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc
dc.relation.ispartofMedical Oncology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectApoptosis
dc.subjectCell viability
dc.subjectInflammation
dc.subjectPervari honey
dc.subjectSH-SY5Y neuroblastoma cells
dc.titleAntiproliferative and apoptotic effects of Pervari honey on SH-SY5Y neuroblastoma cells
dc.typeArticle

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