Effects of somatostatin and vitamin C on the fatty acid profile of breast cancer cell membranes

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dc.contributor.authorHanikoğlu, Ayşegüll
dc.contributor.authorKüçüksayan, Ertan
dc.contributor.authorHanikoğlu, Ferhat
dc.contributor.authorÖzben, Tomris
dc.contributor.authorMenounou, Georgia
dc.contributor.authorSansone, Anna
dc.contributor.authorFerreri, Carla
dc.date.accessioned2021-02-19T21:16:27Z
dc.date.available2021-02-19T21:16:27Z
dc.date.issued2019
dc.departmentALKÜ
dc.descriptionKucuksayan, Ertan/0000-0002-1611-0875
dc.description.abstractBackground: Vitamin C (Vit C) is an important physiological antioxidant with growing applications in cancer. Somatostatin (SST) is a natural peptide with growth inhibitory effect in several mammary cancer models. Objective: The combined effects of SST and Vit C supplementation have never been studied in breast cancer cells so far. Methods: We used MCF-7 and MDA-MB231 breast cancer cells incubated with SST for 24h, in the absence and presence of Vit C, at their EC 50 concentrations, to evaluate membrane fatty acid-profiles together with the follow-up of EGFR and MAPK signaling pathways. Results: The two cell lines gave different membrane reorganization: in MCF-7 cells, decrease of omega-6 linoleic acid and increase of omega-3 fatty acids (Fas) occurred after SST and SST+Vit C incubations, the latter also showing significant increases in MUFA, docosapentaenoic acid and mono-trans arachidonic acid levels. In MDA-MB231 cells, SST+Vit C incubation induced significant membrane remodeling with an increase of stearic acid and mono-trans-linoleic acid isomer, diminution of omega-6 linoleic, arachidonic acid and omega-3 (docosapentaenoic and docosadienoic acids). Distinct signaling pathways in these cell lines were studied: in MCF-7 cells, incubations with SST and Vit C, alone or in combination significantly decreased EGFR and MAPK signaling, whereas in MDA-MB231 cells, SST and Vit C incubations, alone or combined, decreased pP44/42 MAPK levels, and increased EGFR levels. Conclusion: Our results showed that SST and Vit C can be combined to induce membrane fatty acid changes, including lipid isomerization through a specific free radical-driven process, influencing signaling pathways.
dc.description.sponsorshipMarie Sklodowska-Curie Innovative Training Network (ITN) ClickGene [H2020-MSCA-ITN-2014-642023]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [217S253]; Akdeniz University Scientific Research FundsAkdeniz University [TDK-2017-2096]
dc.description.sponsorshipCF, GM, and CC acknowledge funding from the Marie Sklodowska-Curie Innovative Training Network (ITN) ClickGene (H2020-MSCA-ITN-2014-642023).; This study was supported and funded by TUBITAK (Project number: 217S253) and Akdeniz University Scientific Research Funds (TDK-2017-2096).
dc.identifier.doi10.2174/1871520619666190930130732
dc.identifier.endpage1909en_US
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue15en_US
dc.identifier.pmid31566138
dc.identifier.scopusqualityQ2
dc.identifier.startpage1899en_US
dc.identifier.urihttps://doi.org/10.2174/1871520619666190930130732
dc.identifier.urihttps://hdl.handle.net/20.500.12868/433
dc.identifier.volume19en_US
dc.identifier.wosWOS:000506818900011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofAnti-Cancer Agents In Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSomatostatin
dc.subjectVitamin C
dc.subjectmembrane fatty acid profile
dc.subjectbreast cancer
dc.subjectcell signaling
dc.subjectgas chromatography
dc.titleEffects of somatostatin and vitamin C on the fatty acid profile of breast cancer cell membranes
dc.typeArticle

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