Pimecrolimus protects neuron-like SH-SY5Y cells against anti-inflammatory and anti-oxidant effects of both microglial secretome and hydrogen peroxide

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dc.authorid0000-0003-2594-502X
dc.authorid0000-0002-3555-3946
dc.authorid0000-0002-7248-7933
dc.contributor.authorKocanci, Fatma Gonca
dc.contributor.authorErol, Azize Yasemin Goksu
dc.contributor.authorYildiz, Fatma
dc.contributor.authorEciroglu, Hamiyet
dc.date.accessioned2026-01-24T12:29:03Z
dc.date.available2026-01-24T12:29:03Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractCalcineurin inhibitors have been found to exhibit a preventive role against neuroinflammation, which represents a crucial underlying mechanism in neurodegenerative diseases (ND). Additionally, they possess suppressive effects on the activation of apoptotic pathways, which constitute another mechanism underlying such diseases. Given that pimecrolimus, a calcineurin inhibitor, impedes the synthesis of pro-inflammatory cytokines, such as interleukin (IL)-2, IL-4, and IL-10, and influences apoptotic processes, it is noteworthy to test its potential neuroprotective properties. Thus, the objective of this investigation was to assess the potential protective effects of pimecrolimus against the degenerative consequences of both microglial secretomes and hydrogen peroxide (H2O2), an oxidant agent. The survival rates of HMC3 microglia cells, neuron-like differentiated SH-SY5Y (d-SH- SY5Y) cells, and their co-culture were determined using the 3-( 4,5-Dimethylthiazol- 2- yl)- 2,5- Diphenyltetrazolium Bromide (MTT) method. Furthermore, the levels of pro-inflammatory cytokines IL-1 beta and IL-6, and anti-inflammatory cytokine IL-10 were measured using ELISA kits, besides total antioxidant and oxidant capacities in conditioned media of cells. Additionally, the effect of pimecrolimus on neurite length in these cell groups was evaluated through morphological observations. This study revealed, for the first time, that pimecrolimus exerts preventive effects on neurodegenerative processes by virtue of its anti-inflammatory and -antioxidant activities. It holds promise as a potential treatment option for ND.
dc.description.sponsorshipAlanya Keykubat University
dc.description.sponsorshipAlanya Keykubat University
dc.identifier.doi10.1111/sji.13328
dc.identifier.issn0300-9475
dc.identifier.issn1365-3083
dc.identifier.issue1
dc.identifier.pmid38441277
dc.identifier.scopus2-s2.0-85170552436
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/sji.13328
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5099
dc.identifier.volume99
dc.identifier.wosWOS:001067292300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofScandinavian Journal of Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectcalcineurin inhibitor
dc.subjectneural apoptosis
dc.subjectneuroinflammation
dc.subjectneuroprotection
dc.subjectpimecrolimus
dc.titlePimecrolimus protects neuron-like SH-SY5Y cells against anti-inflammatory and anti-oxidant effects of both microglial secretome and hydrogen peroxide
dc.typeArticle

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