The effect of the enzymes trypsin and DNase I on the antimicrobial efficiency of root canal irrigation solutions

Basit Öğe Kaydı
dc.authorid0000-0003-0607-6703
dc.authorid0000-0002-5331-2206
dc.authorid0000-0002-1773-9114
dc.contributor.authorCankaya, Tuelin Dogan
dc.contributor.authorAydin, Zeliha Ugur
dc.contributor.authorErdonmez, Demet
dc.date.accessioned2026-01-24T12:30:55Z
dc.date.available2026-01-24T12:30:55Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractThe purpose of this study was to evaluate the antibacterial efficacy of using 2.5% NaOCl, 2% chlorhexidine (CHX), Irritrol, and chitosan-coated silver nanoparticles (AgCNPs) alone or in combination with deoxyribonuclease I (DNase I) and trypsin pre-enzyme applications in dentin samples contaminated with Enterococcus faecalis (E. faecalis) by CLSM. 144 dentin blocks with confirmed E. faecalis biofilm formation were divided randomly according to the irrigation protocol (n = 12): NaOCl, CHX, Irritrol, AgCNPs, trypsin before NaOCl, CHX, Irritrol, AgCNPs, and DNase I before NaOCl, CHX, Irritrol, AgCNPs. Dentin blocks were stained with the Live/Dead BacLight Bacterial Viability Kit and viewed with CLSM after irrigation applications. The percentage of dead and viable bacteria was calculated using ImageJ software on CLSM images. At a significance level of p < 0.05, the obtained data were analyzed using one-way Anova and post-hoc Tukey tests. In comparison with NaOCl, CHX had a higher percentage of dead bacteria, both when no pre-enzyme was applied and when DNase I was applied as a pre-enzyme (p < 0.05). There was no difference in the percentage of dead bacteria between the irrigation solutions when trypsin was applied as a pre-enzyme (p > 0.05). AgCNPs showed a higher percentage of dead bacteria when trypsin was applied as a pre-enzyme compared to other irrigation solutions (p < 0.05), while the pre-enzyme application did not affect the percentage of dead bacteria in NaOCl, CHX, and Irritrol (p > 0.05). No irrigation protocol tested was able to eliminate the E. faecalis biofilm. While the application of trypsin as a pre-enzyme improved the antimicrobial effect of AgCNPs, it did not make any difference over other irrigation solutions.
dc.description.sponsorshipAbant Izzet Baysal niversitesi
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s10266-023-00894-x
dc.identifier.endpage937
dc.identifier.issn1618-1247
dc.identifier.issn1618-1255
dc.identifier.issue3
dc.identifier.pmid38280114
dc.identifier.scopus2-s2.0-85183401709
dc.identifier.scopusqualityQ1
dc.identifier.startpage929
dc.identifier.urihttps://doi.org/10.1007/s10266-023-00894-x
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5527
dc.identifier.volume112
dc.identifier.wosWOS:001150694500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofOdontology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectDNase I
dc.subjectE. faecalis
dc.subjectIrritrol
dc.subjectSilver chitosan nanoparticle
dc.subjectTrypsin
dc.titleThe effect of the enzymes trypsin and DNase I on the antimicrobial efficiency of root canal irrigation solutions
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu