A novel aptasensor platform for the detection of carcinoembryonic antigen using quartz crystal microbalance

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dc.authorid0000-0003-4702-7457
dc.authorid0000-0002-5550-106X
dc.contributor.authorErkal-Aytemur, Asli
dc.contributor.authorMulazimog, Ibrahim Ender
dc.contributor.authorUstundag, Zafer
dc.contributor.authorCaglayan, Mustafa Oguzhan
dc.date.accessioned2026-01-24T12:31:18Z
dc.date.available2026-01-24T12:31:18Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIn this study, a quartz crystal microbalance (QCM) aptasensor for carcinoembryonic antigen (CEA), a well-known biomarker for various cancer types, was reported, utilizing two different aptamers. To achieve this, a nanofilm of 4-mercaptophenyl was electrochemically attached to gold-coated QCM crystal surfaces via the reduction of 4mercaptobenzenediazonium salt (4 MB-DAT) using cyclic voltammetry. Subsequently, gold nanoparticles (AuNP) were affixed to this structure, and then aptamers (antiCEA1 and antiCEA2) modified with SH-functional ends bound to AuNPs completed the modification. The analytical performance of the CEA sensor was evaluated through simultaneous QCM measurements employing CEA solutions ranging from 0.1 ng/mL to 25 ng/mL. The detection limit (LOD) for CEA was determined to be 102 pg/mL for antiCEA1 and 108 pg/mL for antiCEA2 aptamers. Interday and intraday precision and accuracy tests yielded maximum results of 4.3 and + 3.8, respectively, for both aptasensors, as measured by relative standard deviation (RSD%) and relative error (RE%). The kinetic data of the aptasensors resulted in affinity values (KD) of 0.43 +/- 0.14 nM for antiCEA1 and 0.75 +/- 0.42 nM for antiCEA2. These values were lower than the reported values of 3.9 nM and 37.8 nM for both aptamers, respectively. The selectivity of the aptasensor was evaluated by measuring the signal changes caused by alpha-fetoprotein (AFP), cancer antigen (CA-125), and vascular endothelial growth factor (VEGF-165) individually and together at a concentration of 500 ng/mL, resulting in a maximum 4.1 % change, which was comparable to precision and accuracy values reported in the literature. After confirming the selectivity of the aptamers, recovery experiments were conducted using spiked commercial serum samples to simulate real samples, and the lowest recovery value obtained was 95.4 %. It was determined that two different aptasensors could be successfully used for the QCM-based detection of CEA in this study.
dc.description.sponsorshipTUBITAK (Scientific and Technological Research Council of Turkey) [122Z678]; Kutahya Dumlupimath;nar University; Necmettin Erbakan University [221410001]
dc.description.sponsorshipThis work was supported by TUBITAK (Scientific and Technological Research Council of Turkey) by project number 122Z678. Additionally, the authors would like to thank Kutahya Dumlup & imath;nar University and Necmettin Erbakan University for the financial contribution to this study through the BAP project numbered 2022-60 and 221410001, respectively.
dc.identifier.doi10.1016/j.talanta.2024.126376
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid38852341
dc.identifier.scopus2-s2.0-85195322740
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2024.126376
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5796
dc.identifier.volume277
dc.identifier.wosWOS:001251520100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectCarcinoembryonic antigen
dc.subjectElectrochemical modification
dc.subjectQuartz crystal microbalance
dc.subjectAptasensor
dc.titleA novel aptasensor platform for the detection of carcinoembryonic antigen using quartz crystal microbalance
dc.typeArticle

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