Selenium enhances TRPA1 channel-mediated activity of temozolomide in SH-SY5Y neuroblastoma cells

Basit Öğe Kaydı
dc.contributor.authorÖzkal, Birol
dc.contributor.authorÖvey, İshak Suat
dc.date.accessioned2021-02-19T21:16:31Z
dc.date.available2021-02-19T21:16:31Z
dc.date.issued2020
dc.departmentALKÜ
dc.description.abstractPurpose Neuroblastoma is a malignant solid tumor that originates from the sympathetic nervous system in early childhood. Temozolomide is used for treatment in high-risk groups with low treatment response of neuroblastomas. TRPA1 channels in neuroblastoma cells are calcium permeable channels that can be activated by reactive oxygen species (ROT). In this study, we aimed to evaluate the level of activity of temozolomide and selenium in neuroblastoma cells via TRPA1 channels. Method Seven main groups were formed using SH-SY5Y neuroblastoma cells. The control was divided into temozolomide (TMZ) (100 mu M, 24 h), TMZ+SEL+AP18, SEL (sodium selenite, 100 mu M, 24 h), and SEL+AP18 groups. Intergroup calcium signaling, intracellular reactive oxygen species, caspase-3 and caspase-9, and mitochondrial depolarization analyses were performed by channel activation with TRPA1 agonist cinnamaldehyde in all groups. Results Cytosolic calcium concentration, apoptosis, caspase-3 and caspase-9 activation, mitochondrial membrane depolarization, and ROT levels were higher in TMZ (p < 0.001), TMZ+SEL (p < 0.001), and SEL (p < 0.05) groups than the control group. TRPA1 was lower in TTMZ+AP18, TMZ+SEL+AP18, and SEL+AP18 groups with channel blockers than respectively TMZ, TMZ+SEL, and SEL groups without channel blockers (p < 0.05). Conclusion The use of selenium with temozolomide increased the apoptotic efficacy of temozolomide via TRPA1 channels on tumor cells.
dc.identifier.doi10.1007/s00381-020-04567-w
dc.identifier.endpage1292en_US
dc.identifier.issn0256-7040
dc.identifier.issn1433-0350
dc.identifier.issue6en_US
dc.identifier.pmid32146544
dc.identifier.scopusqualityQ2
dc.identifier.startpage1283en_US
dc.identifier.urihttps://doi.org/10.1007/s00381-020-04567-w
dc.identifier.urihttps://hdl.handle.net/20.500.12868/460
dc.identifier.volume36en_US
dc.identifier.wosWOS:000522713300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofChilds Nervous System
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTemozolomide
dc.subjectNeuroblastoma
dc.subjectOxidative stress
dc.subjectTRPA1
dc.subjectSH-SY5Y
dc.subjectSelenium
dc.titleSelenium enhances TRPA1 channel-mediated activity of temozolomide in SH-SY5Y neuroblastoma cells
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu