The identification of acinetobacter baumannii blood isolates by MALDI-TOF MS, ARDRA and bla(OXA-51-like) Gene-Specific Real-Time PCR

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dc.contributor.authorGözalan, Ayşegül
dc.contributor.authorAydoğan, Sibel
dc.contributor.authorHacıseyitoğlu, Demet
dc.contributor.authorKuzucu, Çiğdem
dc.contributor.authorKöksal, Fatma
dc.contributor.authorAçıkgöz, Ziya Cibali
dc.contributor.authorDurmaz, Rıza
dc.date.accessioned2021-02-19T21:16:10Z
dc.date.available2021-02-19T21:16:10Z
dc.date.issued2020
dc.departmentALKÜ
dc.description.abstractThe Acinetobacter cakoaceticus-Acinetobacter baumannii (Acb) complex consists of phenotypically very similar nosocomial species; A.baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, Acinetobacter seifertii and Acinetobacter djikshoorniae and one environmental species Acakoaceticus. The rapid and accurate identification of the members of Acb complex is critical as these nosocomial pathogens can show differences in antimicrobial susceptibility and clinical outcomes. The conventional phenotypic methods are slow, unreliable and less efficient for the differentiation of Acb complex species, including the A.baumannii species within the Acb complex. Although various molecular methods are available, such as amplified ribosomal DNA restriction analysis (ARDRA) and bla(OXA-51-like), gene specific PCR, they are usually inconvenient for the routine diagnostic laboratories. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) offers an opportunity for rapid, cost-effective and convenient bacterial identification in routine diagnostic procedures conducted in clinical laboratory. In this study, we aimed to evaluate the diagnosis performance of MALDI-TOF MS system to identify blood isolates of A.baumannii. A total of 180 nonduplicate carbapenem resistant Acb complex (strain numbers; TR1-TR60) and A.baumannii (TR61-TR180) blood isolates were collected from the intensive care units of the three university hospitals in Turkey from January 2016 to December 2016. All isolates were evaluated by using bla(OXA-51-like) gene specific real time (Rt-PCR) analysis, ARDRA (restriction enzymes-AIM, Cfol, Mbol, Mspl, Rsal) method and MALDI-TOF MS (VITEK (R) MS, bioMerieux, France) system. All the strains except TRIO, TR31, TR35 and TR52 were identified as A.baumannii by ARDRA method. Out of 177 of all the isolates, presence of bla(OXA-51-like) gene was found except for TR10, TR31 and TR52 isolates. However, TR31 without the presence of bla(OXA-51-like) gene was identified as A.pittii using the ARDRA. Totally 176 isolates which were identified as A. baumannii by both of the methods, ARDRA and Rt-PCR-bla(OXA-51-like) ,were accepted as a reference for the evaluation of the diagnosis performance capacity of the MALDI-TOF MS. Overall, for all 176 isolates tested, the sensitivity obtained with the MALDI-TOF MS were 99.4% with 75% specificity. The accuracy value of the method was determined as 98.9% for the identification of A.baumannii to the species level. MALDI-TOF MS is increasingly used in diagnostic microbiology for the routine identification of bacteria to the genus, species or subspecies level with high rates of sensitivity and specificity. In future, by expanding the database, MALDI-TOF MS system would possibly become the ideal method for routine diagnostic laboratories that could potentially identify more species and even determine some characteristics of antimicrobial resistance and virulence determinants.
dc.identifier.doi10.5578/mb.70085
dc.identifier.endpage546en_US
dc.identifier.issn0374-9096
dc.identifier.issue4en_US
dc.identifier.pmid33107283
dc.identifier.scopusqualityQ3
dc.identifier.startpage535en_US
dc.identifier.urihttps://doi.org/10.5578/mb.70085
dc.identifier.urihttps://hdl.handle.net/20.500.12868/281
dc.identifier.volume54en_US
dc.identifier.wosWOS:000582397200002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isotr
dc.publisherAnkara Microbiology Soc
dc.relation.ispartofMikrobiyoloji Bulteni
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcinetobacter baumannii
dc.subjectpolymerase chain reaction
dc.subjectamplified ribosomal DNA restriction analysis
dc.subjectmatrix-assisted laser desorption-ionization
dc.subjectmass spectrometry
dc.titleThe identification of acinetobacter baumannii blood isolates by MALDI-TOF MS, ARDRA and bla(OXA-51-like) Gene-Specific Real-Time PCR
dc.typeArticle

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