Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Blood Culture Isolates from Three Hospitals in Turkey

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dc.authorid0000-0001-6561-778X
dc.authorid0000-0002-0436-7550
dc.contributor.authorGozalan, Aysegul
dc.contributor.authorUnaldi, Ozlem
dc.contributor.authorGuldemir, Dilek
dc.contributor.authorAydogan, Sibel
dc.contributor.authorKuzucu, Cigdem
dc.contributor.authorCakirlar, Fatma Koksal
dc.contributor.authorAcikgoz, Ziya Cibali
dc.date.accessioned2026-01-24T12:26:52Z
dc.date.available2026-01-24T12:26:52Z
dc.date.issued2021
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractWe aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals in Turkey in 2016. Molecular epidemiological characteristics of the isolates were examined using pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, and rpoB). Multiplex PCR was used to investigate the carbapenemase genes, including bla(OXA-23-like),bla(OXA-24-like), bla(OXA-48-like), bla(OXA-58-like), bla(IMP), bla(VIM) and bla(NDm). PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. As per a >= 85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The bla(OXA-)(23-like) and bla(OXA-)(58)-like genes were identified in 100% and 28.2% of the isolates, respectively. The bla(NDM) gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes, and 29 sequence types (STs). Most of the isolates (n = 36) belonged to the clonal complex (CC)2, one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were identified. Identifying both global ST2 and a large number of new STs, revealed high genetic diversity in A. baumannii isolates in the study population.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) 3001-Starting R&D Projects Funding Program [116S249]
dc.description.sponsorshipWe thank to The Scientific and Technological Research Council of Turkey (TUBITAK). This study was funded by The Scientific and Technological Research Council of Turkey (TUBITAK) 3001-Starting R&D Projects Funding Program (grant number: 116S249) titled as Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii blood isolates: Multilocus Sequence Typing, Pulsotyping, and Resistance-Related Mutation Analysis.
dc.identifier.doi10.7883/yoken.JJID.2020.478
dc.identifier.endpage208
dc.identifier.issn1344-6304
dc.identifier.issn1884-2836
dc.identifier.issue3
dc.identifier.pmid33250488
dc.identifier.scopus2-s2.0-85107088652
dc.identifier.scopusqualityQ3
dc.identifier.startpage200
dc.identifier.urihttps://doi.org/10.7883/yoken.JJID.2020.478
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4967
dc.identifier.volume74
dc.identifier.wosWOS:000668203500005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNatl Inst Infectious Diseases
dc.relation.ispartofJapanese Journal of Infectious Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectEscherichia-Coli
dc.subjectBeta-Lactamases
dc.subjectAntimicrobial Resistance
dc.subjectMediterranean Countries
dc.subjectHigh Prevalence
dc.subjectMultiplex Pcr
dc.subjectIdentification
dc.subjectGenes
dc.subjectInfections
dc.subjectDiversity
dc.titleMolecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Blood Culture Isolates from Three Hospitals in Turkey
dc.typeArticle

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