Increased oxidative and chromosomal DNA damage in patients with ankylosing spondylitis: its role in pathogenesis

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dc.authorid0000-0001-8429-009X
dc.authorid0000-0002-1290-9540
dc.authorid0000-0002-3555-3946
dc.authorid0000-0001-6473-5813
dc.contributor.authorKiranatlioglu-Firat, Funda
dc.contributor.authorDemir, Huseyin
dc.contributor.authorCuce, Isa
dc.contributor.authorAltin-Celik, Pinar
dc.contributor.authorEciroglu, Hamiyet
dc.contributor.authorBayram, Fahri
dc.contributor.authorDonmez-Altuntas, Hamiyet
dc.date.accessioned2026-01-24T12:30:55Z
dc.date.available2026-01-24T12:30:55Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIncreased DNA damage has been suggested to contribute to the pathogenesis of chronic inflammatory diseases, but controlled studies are lacking in ankylosing spondylitis (AS). Therefore, we assessed oxidative stress, oxidative DNA damage, chromosomal DNA damage, cell proliferation and cell death in the peripheral blood lymphocytes of patients with AS as well as the possible role of DNA damage in the development of the disease. In total, 25 newly diagnosed AS patients who had not received anti-inflammatory agents and 25 healthy controls were recruited. Oxidative DNA damage was assessed by plasma 8-hydroxy-2 '-deoxyguanosine (8-OHdG) levels, and chromosomal DNA damage was assessed by the cytokinesis-block micronucleus cytome (CBMN-cyt) method. Compared to controls, the micronucleus (MN) frequencies, nucleoplasmic bridge (NPB) frequencies, nuclear bud (NBUD) frequencies, apoptotic cell frequencies, necrotic cell frequencies and plasma 8-OHdG levels were significantly higher in patients with AS (p < 0.001, p < 0.05, p < 0.01, p < 0.001, p < 0.001, and p < 0.001, respectively), and the metaphase cell numbers, binucleated (BN) cell frequencies and nuclear division index (NDI) values were significantly lower in patients with AS (p < 0.01, p < 0.001 and p < 0.001, respectively). Thus, the present findings suggested that oxidative stress, oxidative DNA damage, and chromosomal DNA damage may be involved in the pathogenesis of AS similar to other chronic inflammatory diseases. In addition, the increased plasma 8-OHdG levels, MN frequencies, NPB frequencies and NBUD frequencies in AS patients may reflect an increased cancer risk.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Erciyes University [TTU-2018-8485]
dc.description.sponsorshipThis study was financially supported by Scientific Research Projects Coordination Unit of Erciyes University (Project Code: TTU-2018-8485).
dc.identifier.doi10.1007/s10238-022-00957-3
dc.identifier.endpage1728
dc.identifier.issn1591-8890
dc.identifier.issn1591-9528
dc.identifier.issue5
dc.identifier.pmid36441439
dc.identifier.scopus2-s2.0-85142894318
dc.identifier.scopusqualityQ2
dc.identifier.startpage1721
dc.identifier.urihttps://doi.org/10.1007/s10238-022-00957-3
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5526
dc.identifier.volume23
dc.identifier.wosWOS:000889397600002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer-Verlag Italia Srl
dc.relation.ispartofClinical and Experimental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectAnkylosing spondylitis
dc.subjectCBMN cytome method
dc.subjectDNA damage
dc.subjectMicronucleus
dc.subject8-OHdG levels
dc.subjectOxidative stress
dc.titleIncreased oxidative and chromosomal DNA damage in patients with ankylosing spondylitis: its role in pathogenesis
dc.typeArticle

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