Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production

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dc.authorid0000-0003-4599-6908
dc.authorid0000-0002-3052-4556
dc.contributor.authorIncir, Ibrahim
dc.contributor.authorKaplan, Ozlem
dc.date.accessioned2026-01-24T12:31:17Z
dc.date.available2026-01-24T12:31:17Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractE. coli plays a substantial role in recombinant protein production. Its importance increased with the discovery of recombinant DNA technology and the subsequent production of the first recombinant insulin in E. coli. E. coli is a widely used and cost-effective host to produce recombinant proteins. It is also noteworthy that a significant portion of the approved therapeutic proteins have been produced in this organism. Despite these advantages, it has some disadvantages, such as toxicity and lack of eukaryotic post-translational modifications that can lead to the production of misfolded, insoluble, or dysfunctional proteins. This study focused on the challenges and engineering approaches for improved expression and solubility in recombinant protein production in E. coli. In this context, solution strategies such as strain and vector selection, codon usage, mRNA stability, expression conditions, translocation to the periplasmic region and addition of fusion tags in E. coli were discussed.
dc.identifier.doi10.1016/j.pep.2024.106463
dc.identifier.issn1046-5928
dc.identifier.issn1096-0279
dc.identifier.pmid38479588
dc.identifier.scopus2-s2.0-85187644481
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.pep.2024.106463
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5777
dc.identifier.volume219
dc.identifier.wosWOS:001222241900001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofProtein Expression and Purification
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectE. coli
dc.subjectRecombinant protein
dc.subjectOptimized protein production
dc.subjectSolubility of protein
dc.titleEscherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production
dc.typeReview Article

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