A rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesis

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dc.authorid0000-0002-2316-5495
dc.authorid0000-0002-5435-2074
dc.contributor.authorNamli Kalem, Muberra
dc.contributor.authorAnadol, Elvan
dc.contributor.authorKalem, Ziya
dc.contributor.authorSezginer, Perihan Yalcinkaya
dc.contributor.authorElmas, Cigdem
dc.contributor.authorYilmaz, Canan
dc.contributor.authorBakirarar, Batuhan
dc.date.accessioned2026-01-24T12:31:21Z
dc.date.available2026-01-24T12:31:21Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractThe objective of this investigation was to examine alterations in PTEN expression within ovarian tissue in a rat model of polycystic ovary syndrome (PCOS). The analysis also encompassed the examination of PTEN alterations in the ovarian tissue throughout the process of folliculogenesis in rats with normal ovulatory cycles. The study involved 12 adult female Sprague-Dawley rats randomly assigned to the letrozole-induced polycystic ovary syndrome (PCOS) group as part of an animal-based research endeavour. The sections derived from the ovaries were subjected to immunohistochemical staining for PTEN. The evaluation of PTEN staining levels in ovarian tissues was conducted using electron microscopy. Follicle counts, as well as hormonal and biochemical analyses (serum luteinising hormone (LH), follicle-stimulating hormone (FSH), anti-Mullerian hormone (AMH), testosterone, oestradiol levels and serum glucose, triglyceride, HDL and LDL-cholesterol levels), were conducted to provide evidence of the manifestation of polycystic ovary syndrome (PCOS) in rats. The number of primordial and Graafian follicles in the PCOS group decreased significantly, and the number of primary, secondary and antral follicles increased significantly. PTEN expression was found to be significantly higher in the PCOS group than in the control group in the primordial follicle oocyte cytoplasm, primordial follicle granulosa cells, primary follicle oocyte cytoplasm, primary follicle granulosa cells, antral follicle oocyte cytoplasm, antral follicle granulosa cells, and corpus luteum (p = 0.007, p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.002, and p = 0.018, respectively). In the non-PCOS group, a time-dependent comparison of the amount of oocyte cytoplasm and PTEN staining in granulosa cells of the oocytes at different stages of development was performed. While the follicles were developing from the primordial follicle to the primary and antral follicle, the amount of PTEN staining in the oocyte cytoplasm decreased, whereas the PTEN activity in the granulosa cells increased as the oocyte developed (p = 0.001 and p = 0.001, respectively). The current investigation demonstrated changes in PTEN expression in ovarian tissue throughout the course of normal folliculogenesis, as well as in instances of disrupted folliculogenesis, with a focus on rats with PCOS.
dc.identifier.doi10.1038/s41598-023-47809-y
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid38008769
dc.identifier.scopus2-s2.0-85177731850
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-023-47809-y
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5827
dc.identifier.volume13
dc.identifier.wosWOS:001124265200003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectGranulosa-Cells
dc.subjectPathway
dc.subjectGrowth
dc.subjectDifferentiation
dc.subjectProliferation
dc.subjectFsh
dc.titleA rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesis
dc.typeArticle

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