Evaluation of caffeic acid fenethyl ester in testicular damage caused by fluoxetine hydrochloride in rats: effects of fluoxetine hydrochloride on testes

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dc.contributor.authorAsoglu, Zehra Demiray
dc.contributor.authorBalli, Ebru
dc.contributor.authorBayrak, Gulsen
dc.contributor.authorYalin, Serap
dc.contributor.authorOzturk, Ayla Batu
dc.contributor.authorKaya, Merih Akkapulu
dc.contributor.authorSengul, Merve Turkegun
dc.date.accessioned2026-01-24T12:30:52Z
dc.date.available2026-01-24T12:30:52Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIn our study, the aim was to investigate the protective effect of caffeic acid phenethyl ester, which has a wide spectrum of activity, against testicular damage caused by fluoxetine hydrochloride. In our study, 25 male Wistar Albino rats were used, and the rats were divided into five groups with an equal number of rats in each group: control 1 (saline), control 2 (dimethyl sulfoxide-DMSO), fluoxetine group (FLX), caffeic acid phenethyl ester group (CAPE), and fluoxetine + caffeic acid phenethyl ester group (FLX + CAPE). After a 4-week experiment, blood samples were collected, and testicular tissues were excised. For light microscopic evaluation, hematoxylin-eosin (H&E) and toluidine blue (TB) staining were performed. Immunohistochemical examinations were carried out with caspase-3, Bcl-2, and PCNA immunostaining. As a result of statistical evaluation of the morphologic and immunohistochemical examinations, it was determined tubule diameter (p < 0.001, eta(2) = 0.798), germinal epithelium thickness (p = 0.0001, eta(2) = 0.573), and Johnsen score (p < 0.001, eta(2) = 0.928) decreased in the FLX group compared to all groups, while these valuesincreased in the FLX + CAPE group compared to the FLX group. Similarly, body weight and right testicular weight too. Caspase-3 expression in Leydig cells and Bcl-2 expression in seminiferous tubules were increased in FLX group compared to SF, DMSO, and CAPE groups, while they were decreased in FLX + CAPE group compared to FLX group. PCNA index was decreased in FLX group compared to SF, DMSO, and CAPE groups, while it was increased in FLX + CAPE group compared to FLX group. In biochemical analyses, MDA level was increased in the FLX group, while this value was decreased in the FLX + CAPE group. No apparent change was observed in SOD and CAT levels. Our findings showed that FLX induced apoptosis by causing structural damage in the testis through oxidative effects, while CAPE, with its antioxidant effect, repaired this damage, reducing lipid peroxidation and oxidative stress.
dc.description.sponsorshipMersin University Scientific Research Projects Unit [2021-1-TP2-4298]
dc.description.sponsorshipThis study was carried out with the financial support provided by Mersin University Scientific Research Projects Unit within the scope of project number 2021-1-TP2-4298.
dc.identifier.doi10.1007/s00210-025-04849-7
dc.identifier.issn0028-1298
dc.identifier.issn1432-1912
dc.identifier.pmid41389347
dc.identifier.scopus2-s2.0-105024808708
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s00210-025-04849-7
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5500
dc.identifier.wosWOS:001638823800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofNaunyn-Schmiedebergs Archives of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectApoptosis
dc.subjectCAPE
dc.subjectFluoxetine
dc.subjectImmunohistochemistry
dc.subjectTestes
dc.titleEvaluation of caffeic acid fenethyl ester in testicular damage caused by fluoxetine hydrochloride in rats: effects of fluoxetine hydrochloride on testes
dc.typeArticle

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