Coffee induces AHR- and Nrf2-mediated transcription in intestinal epithelial cells

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dc.contributor.authorToydemir, Gamze
dc.contributor.authorLoonen, Linda M. P.
dc.contributor.authorVenkatasubramanian, Prashanna Balaji
dc.contributor.authorMes, Jurriaan J.
dc.contributor.authorWells, Jerry M.
dc.contributor.authorDe Wit, Nicole
dc.date.accessioned2021-02-19T21:16:27Z
dc.date.available2021-02-19T21:16:27Z
dc.date.issued2021
dc.departmentALKÜ
dc.description.abstractCoffee induces a health-promoting adaptive response of cells in the body. Here, we investigated enterocyte responses to AHR agonists in coffee and measured their transport across a polarized intestinal epithelium. AHR-activating potencies of Turkish, filter, and instant coffee were determined using DR CALUX (R) bioassay, before and after intestinal metabolization by Caco-2 cells. Furthermore, effects of coffee on induction of AHRand Nrf2-pathway genes in Caco-2 cells were evaluated by real-time qPCR. Coffee samples showed considerable AHRactivating potencies in DR CALUX (R) bioassay (up to 79% of positive control activity). After incubation with Caco2 cells, AHR activity of different coffees was between 35 and 64% of their initial value, suggesting rapid uptake and metabolization by epithelial cells. Expression of AHR-regulated gene CYP1A1 increased up to 41-fold and most Nrf2-pathway genes were up-regulated by coffee. This in vitro study may support the notion that coffee bioactives contribute to antioxidant defense and detoxification processes in vivo.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Dutch Ministry of Economic Affairs within the Systems Biology programme `Virtual Gut' [KB-17-003.02-021]
dc.description.sponsorshipThe authors kindly thank Renata Ariens and Monic Tomassen for analytical support. This work was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) and by the Dutch Ministry of Economic Affairs within the Systems Biology programme `Virtual Gut', KB-17-003.02-021. The funding sources were not involved in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.
dc.identifier.doi10.1016/j.foodchem.2020.128261
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.pmid33038802
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2020.128261
dc.identifier.urihttps://hdl.handle.net/20.500.12868/436
dc.identifier.volume341en_US
dc.identifier.wosWOS:000602307500007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCoffee
dc.subjectAHR
dc.subjectNrf2
dc.subjectDR CALUX (R)
dc.subjectCaco-2
dc.subjectReal-time qPCR
dc.titleCoffee induces AHR- and Nrf2-mediated transcription in intestinal epithelial cells
dc.typeArticle

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