Minocycline increases in-vitro cortical neuronal cell survival after laser induced axotomy

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dc.contributor.authorYuluğ, Burak
dc.contributor.authorOzansoy, Mehmet
dc.contributor.authorAlökten, Merve
dc.contributor.authorOzansoy, Muzaffer
dc.contributor.authorÇankaya, Şeyda
dc.contributor.authorHanoğlu, Lütfü
dc.contributor.authorKılıç, Ertuğrul
dc.date.accessioned2021-02-19T21:16:31Z
dc.date.available2021-02-19T21:16:31Z
dc.date.issued2020
dc.departmentALKÜ
dc.descriptionAlokten, Merve/0000-0002-5955-5356; Cankaya, Seyda/0000-0001-5309-0351; Kilic, Ertugrul/0000-0001-6494-8923
dc.description.abstractBackground: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical application of such candidate molecules when it comes to human patients. Methods: After 24 hours of culturing, three different minocycline (Sigma-Aldrich, M9511, Germany) concentrations (1 mu M, 10 mu M and 100 mu M) were added to the primary cortical neurons 15 minutes before laser axotomy procedure in order to observe protective effect of minocycline in these dosages. Results: Here, we have shown that minocycline exerted a significant neuroprotective effect at 1 and 100 mu M doses. Beyond confirming the neuroprotective effect of minocycline in a more standardised and advanced in-vitro trauma model, our findings could have important implications for future studies that concentrate on the translational block between animal and human studies. Conclusion: Such sophisticated approaches might also help to conquer the influence of humanmade variabilities in critical experimental injury models. To the best of our knowledge, this is the first study showing that minocycline increases in-vitro neuronal cell survival after laser-axotomy.
dc.identifier.doi10.2174/1574884714666190226093119
dc.identifier.endpage109en_US
dc.identifier.issn1574-8847
dc.identifier.issn2212-3938
dc.identifier.issue2en_US
dc.identifier.pmid30813881
dc.identifier.scopusqualityN/A
dc.identifier.startpage105en_US
dc.identifier.urihttps://doi.org/10.2174/1574884714666190226093119
dc.identifier.urihttps://hdl.handle.net/20.500.12868/461
dc.identifier.volume15en_US
dc.identifier.wosWOS:000579880000003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCurrent Clinical Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMinocycline
dc.subjectlaser-axotomy
dc.subjectin-vitro cortical cell culture
dc.subjecttranslational neuroscience
dc.subjecttrauma
dc.subjectpropidium
dc.titleMinocycline increases in-vitro cortical neuronal cell survival after laser induced axotomy
dc.typeArticle

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