PolyPyrrole based-impedimetric aptasensor for selective determination of beta-HCG from urine sample

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dc.authorid0000-0003-1421-9962
dc.authorid0000-0002-3971-7725
dc.authorid0000-0001-5564-5883
dc.contributor.authorDeniz, Alparslan
dc.contributor.authorKarasu, Tunca
dc.contributor.authorOzgur, Erdogan
dc.contributor.authorUzun, Lokman
dc.date.accessioned2026-01-24T12:31:07Z
dc.date.available2026-01-24T12:31:07Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractHerein, a conjugated conducting polymer-based impedimetric aptasensor has been developed to detect beta-human chorionic gonadotropin (bHCG), the one of the important biomarkers in gynecology, from synthetic human urine samples. In this context, gold electrodes were, firstly coated with pyrrole and pyrrole-3-carboxylic acid to obtain the poly(pyrrole-pyrrole-3-carboxylic acid) [poly(Py-PyCOOH)] conductive copolymer by cyclic voltammetry (CV). Then, bHCG-specific peptide aptamer was covalently linked onto the surface via applying a well-known carbodiimide-succinimide chemistry. The sensor developed was characterized to confirm modification steps via both electrochemical methods including CV, electrochemical impedance spectroscopy (EIS), and chronoamperometry and physico-chemically via attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), atomic force microscope (AFM), and contact angle measurements (CA). The analytical performance of the sensor was evaluated in the concentration range from 1 mu g/mL to 100 mu g/mL for successful detection of bHCG even in the presence of interference agents. The results have also revealed that the sensor could be classified as a promising alternative to its benchmark commercial clinical methods due its superior properties such as cost-friendliness, easy-to-prepare, stable, robust, and selectivity / sensitivity.
dc.identifier.doi10.1016/j.bioelechem.2024.108820
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid39299186
dc.identifier.scopus2-s2.0-85204093537
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2024.108820
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5648
dc.identifier.volume161
dc.identifier.wosWOS:001320215000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofBioelectrochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectPolypyrrole
dc.subjectAptasensor
dc.subjectHuman chorionic gonadotropin
dc.subjectImpedimetric detection
dc.subjectUrine
dc.titlePolyPyrrole based-impedimetric aptasensor for selective determination of beta-HCG from urine sample
dc.typeArticle

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