Evaluation of fisetin as a potential inducer of mitochondrial biogenesis in SH-SY5Y neuronal cells

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dc.contributor.authorAy, Muhammet
dc.date.accessioned2026-01-24T12:29:20Z
dc.date.available2026-01-24T12:29:20Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractObjective(s): Increasing evidence implicates impaired mitochondrial biogenesis as an important contributor to mitochondrial dysfunction, which plays a central role in the pathogenesis of neurodegenerative diseases including Parkinson's disease (PD). For this reason, targeting mitochondrial biogenesis may present a promising therapeutic strategy for PD. The present study attempted to investigate the effects of fisetin, a dietary flavonoid, on mitochondrial biogenesis and the expression of PD-associated genes in neuronal cells. Materials and Methods: The effects of fisetin on mitochondrial biogenesis were evaluated by three different approaches; PGC-1 alpha and TFAM mRNA expressions by RT-qPCR, mitochondrial DNA (mtDNA) content by quantitative PCR and mitochondrial mass by MitoTracker staining. Next, a PCR array was performed to evaluate the effects of fisetin on the expression profile of PD-associated genes. Finally, the common targets of fisetin and PD were analyzed by in silico analyses. Results: The results demonstrated that fisetin treatment can increase PGC-1 alpha and TFAM mRNA levels, mtDNA copy number, and mitochondrial mass in neuronal cells. Fisetin also altered the expressions of some PD-related genes involved in neuroprotection and neuronal differentiation. Moreover, the bioinformatics analyses suggested that the AKT1-GSK3B signaling might be responsible for the potential neuroprotective effects of fisetin. Conclusion: Collectively, these results imply that fisetin could modulate some neuroprotective mechanisms including mitochondrial biogenesis, and may serve as a potential drug candidate for PD.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [118S548]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) , Project No: 118S548.
dc.identifier.doi10.22038/IJBMS.2023.72272.15714
dc.identifier.endpage1325
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue11
dc.identifier.pmid37886009
dc.identifier.scopus2-s2.0-85179393243
dc.identifier.scopusqualityQ2
dc.identifier.startpage1320
dc.identifier.urihttps://doi.org/10.22038/IJBMS.2023.72272.15714
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5295
dc.identifier.volume26
dc.identifier.wosWOS:001076539200009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMashhad Univ Med Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectMitochondria
dc.subjectParkinson's disease
dc.subjectPCR
dc.subjectSH-SY5Y
dc.subjectFisetin
dc.subjectmtDNA
dc.titleEvaluation of fisetin as a potential inducer of mitochondrial biogenesis in SH-SY5Y neuronal cells
dc.typeArticle

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