The increased chromosomal DNA damage in patients with Familial Mediterranean Fever

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dc.authorid0000-0002-3555-3946
dc.authorid0000-0001-7317-2717
dc.authorid0000-0001-6473-5813
dc.contributor.authorKiraz, Aslihan
dc.contributor.authorEciroglu, Hamiyet
dc.contributor.authorAltin-Celik, Pinar
dc.contributor.authorDonmez-Altuntas, Hamiyet
dc.date.accessioned2026-01-24T12:31:26Z
dc.date.available2026-01-24T12:31:26Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractFamilial Mediterranean Fever (FMF) is an inherited autoinflammatory disease. In this study, we aimed to assess chromosomal DNA damage and cell proliferation by using cytokinesis-block micronucleus cytome (CBMN-cyt) assay in the peripheral blood lymphocytes of untreated FMF patients carrying M694V and R202Q mutations, which are the most common MEFV gene mutations in Turkish society. The study included 20 untreated FMF patients with M694V and R202Q mutations and 20 healthy individuals of similar age and sex as the control group. Micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) were scored in the obtained bi-nucleated (BN) cells. Additionally, the nuclear division index (NDI) was calculated using the scores of mononuclear, binuclear, and multinuclear cells. We found that MN and NPBs frequencies in FMF patients were significantly higher than in controls, and number of metaphases was significantly lower (respectively, p < 0.05, p < 0.01, and p < 0.01). However, there was no significant difference in NBUDs frequencies and NDI values between FMF patients and controls (p > 0.05). Our study is the first to evaluate FMF patients' lymphocytes using the CBMN-cyt assay, as no previous research has been found in this respect. Increased MN and NPB frequencies may be useful as biomarkers for chromosomal DNA damage, and may indicate a potential for elevated cancer risk in untreated FMF patients.
dc.description.sponsorshipno specific [2018/526]; Helsinki Declaration of the World Medical Association (Erciyes University Clinical Research Ethics Committee)
dc.description.sponsorshipMany thanks to patients and their families who participated in the study. The authors declare that the procedures were followed according to the regulations established by the Clinical Research and Ethics Committee and to the 2013 Helsinki Declaration of the World Medical Association (Erciyes University Clinical Research Ethics Committee, decision number: 2018/526).
dc.identifier.doi10.1080/10520295.2024.2383960
dc.identifier.endpage312
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue6
dc.identifier.pmid39092615
dc.identifier.scopus2-s2.0-85200258602
dc.identifier.scopusqualityQ2
dc.identifier.startpage305
dc.identifier.urihttps://doi.org/10.1080/10520295.2024.2383960
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5887
dc.identifier.volume99
dc.identifier.wosWOS:001283397000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectCBMN-cyt assay
dc.subjectDNA damage
dc.subjectFamilial Mediterranean Fever (FMF)
dc.subjectMicronucleus (MN)
dc.subjectnucleoplasmic bridges (NPBs)
dc.titleThe increased chromosomal DNA damage in patients with Familial Mediterranean Fever
dc.typeArticle

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