Synergic and comparative effect of 5-fluorouracil and leucoverin on breast and colon cancer cells through TRPM2 channels

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dc.contributor.authorGüler, Yılmaz
dc.contributor.authorÖvey, İshak Suat
dc.date.accessioned2021-02-19T21:16:30Z
dc.date.available2021-02-19T21:16:30Z
dc.date.issued2018
dc.departmentALKÜ
dc.description.abstractOBJECTIVES: We aimed to reveal the role of 5-fluorouracil (5-FU) and Leucovorin (LV) along with transient receptor potential protein melastatin 2 (TRPM2) channels in breast and colon cancer cells during the treatment process. BACKGROUND: 5-FU and LV are widely used in breast and colon cancers for chemotherapy. It has been reported that the expression of TRPM2 channels increased intensively in cancer cells. METHODS: Breast (MCF7) and colon (Caco-2) cells were cultured and divided into seven main groups. The cells in the group were incubated with 5-FU and LV for 24 hrs and then incubated with Antranilic acid. The effects of medicines were investigated on all molecular pathways of apoptosis. RESULTS: It was found that 5FU and LCV, administered separately and together on breast cancer cell culture and colon cancer cell culture increased the intracellular calcium levels by stimulation of TRPM2 channels in both cancer cells. CONCLUSION: As the result of our study, it has been shown that apoptotic effects of 5FU and LV on both colon and breast cancer cells were directly related to TRPM2 channels and that TRPM2 channels played an important role in the whole molecular pathway of apoptosis leading to increased intracellular Ca2+ (Ca2+) levels and increased mitochondrial depolarisation (Fig. 6, Ref. 43). Text in PDF www.elis.sk.
dc.identifier.doi10.4149/BLL_2018_124
dc.identifier.endpage700en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue11en_US
dc.identifier.pmid30686002
dc.identifier.scopusqualityQ2
dc.identifier.startpage692en_US
dc.identifier.urihttps://doi.org/10.4149/BLL_2018_124
dc.identifier.urihttps://hdl.handle.net/20.500.12868/455
dc.identifier.volume119en_US
dc.identifier.wosWOS:000453415200004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherComenius Univ
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbreast and colorectal cancers
dc.subjectTRPM2 channel
dc.subject5-flourouracil
dc.subjectleucoverin
dc.subjectoxidative stress
dc.titleSynergic and comparative effect of 5-fluorouracil and leucoverin on breast and colon cancer cells through TRPM2 channels
dc.typeArticle

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