Altered expression of Notch signaling, Tlr receptors, and surfactant protein expression after prostaglandin inhibition may be associated with the delayed labor in LPS-induced mice

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dc.authorid0000-0003-0370-8680
dc.authorid0000-0002-2860-5592
dc.contributor.authorAvci, Sema
dc.contributor.authorKuscu, Nilay
dc.contributor.authorDurkut, Begum
dc.contributor.authorKilinc, Leyla
dc.contributor.authorUstunel, Ismail
dc.contributor.authorCelik-Ozenci, Ciler
dc.date.accessioned2026-01-24T12:30:58Z
dc.date.available2026-01-24T12:30:58Z
dc.date.issued2022
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractPurpose This study aims to investigate whether indomethacin (IND) delays preterm birth by regulating the Notch pathway, Tlr receptors, and Sp-A in the placenta in lipopolysaccharide (LPS)-induced preterm labor (PTL) model. Methods CD-1 mice were distributed to the pregnant control (PC), Sham, PBS, IND (2 mg/kg; i.p.), LPS (25 mu g/100 mu l; intrauterine), and LPS + IND groups. The injections were performed on day 14.5 of pregnancy. Placentae were collected on day 15.5 of pregnancy, and immunohistochemical analyzes were performed. Differences in staining intensities between the Cox-1, Notch-1 (N1), Dll-1, Jagged-2 (Jag-2), Tlr-2, and Tlr-4 proteins were compared. Results Preterm labor rates were 100% and 66% (preterm delivery delayed 5 h) in the LPS and LPS + IND groups, respectively. In LPS-treated mice, a general morphological deterioration was observed in the placenta. Total placental mid-sagittal measurement was significantly reduced in the LPS-treated group, while it was similar to the PC group in the LPS + IND group. Cox-1 expression in the LZ increased, and Sp-A expression decreased after LPS injection, and IND administration diminished this increase. N1 expression increased in the labyrinth zone (LZ) and the junctional zone (JZ). Dll-1 and Jag-2 expression increased in the JZ after LPS injection (p < 0.0001). IND administration diminished Tlr-2 expression in the LZ and Tlr-4 expression in the JZ after LPS injection. Conclusion In conclusion, PG (prostaglandin) inhibition may alter Notch signaling, Tlr, and Sp-A protein expression and may be associated with delayed labor in LPS-induced mice.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [1059B141700505-2214/A]
dc.description.sponsorshipSA was funded by the Scientific and Technological Research Council of Turkey with International Research Fellowship 2214-A Programme for PhD students grant (1059B141700505-2214/A. The study represents a part of the PhD thesis of SA.
dc.identifier.doi10.1007/s10815-022-02515-y
dc.identifier.endpage1544
dc.identifier.issn1058-0468
dc.identifier.issn1573-7330
dc.identifier.issue7
dc.identifier.pmid35538257
dc.identifier.scopus2-s2.0-85129736912
dc.identifier.scopusqualityQ1
dc.identifier.startpage1531
dc.identifier.urihttps://doi.org/10.1007/s10815-022-02515-y
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5544
dc.identifier.volume39
dc.identifier.wosWOS:000792983600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer/Plenum Publishers
dc.relation.ispartofJournal of Assisted Reproduction and Genetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectPreterm birth
dc.subjectNotch signaling
dc.subjectProstaglandin inhibitors
dc.subjectPlacenta
dc.subjectInflammation
dc.titleAltered expression of Notch signaling, Tlr receptors, and surfactant protein expression after prostaglandin inhibition may be associated with the delayed labor in LPS-induced mice
dc.typeArticle

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