Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining

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dc.contributor.authorŞansaçar, Merve
dc.contributor.authorGencer Akçok, Emel Başak
dc.date.accessioned2026-01-24T12:20:45Z
dc.date.available2026-01-24T12:20:45Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractAutophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis. © Springer Science+Business Media, LLC 2024.
dc.identifier.doi10.1007/7651_2024_526
dc.identifier.endpage224
dc.identifier.isbn9781597452946
dc.identifier.isbn9781617792304
dc.identifier.isbn9781617797668
dc.identifier.isbn1597455741
dc.identifier.isbn9781603272476
dc.identifier.isbn9781597453035
dc.identifier.isbn9781493912230
dc.identifier.isbn9781588298645
dc.identifier.isbn9781617793394
dc.identifier.isbn9781617799648
dc.identifier.issn1064-3745
dc.identifier.pmid38446407
dc.identifier.scopus2-s2.0-85219494510
dc.identifier.scopusqualityQ4
dc.identifier.startpage219
dc.identifier.urihttps://doi.org/10.1007/7651_2024_526
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4558
dc.identifier.volume2879
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biology
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20260121
dc.subjectAutophagic compartments
dc.subjectAutophagic flux
dc.subjectAutophagosome
dc.subjectAutophagy
dc.subjectCancer
dc.subjectCyto-ID
dc.subjectFlow cytometry
dc.titleMeasurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining
dc.typeBook Chapter

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