Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining
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Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Humana Press Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis. © Springer Science+Business Media, LLC 2024.
Açıklama
Anahtar Kelimeler
Autophagic compartments, Autophagic flux, Autophagosome, Autophagy, Cancer, Cyto-ID, Flow cytometry
Kaynak
Methods in Molecular Biology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
2879
