The effect of thymoquinone and propranolol combination on epidermoid laryngeal carcinoma cell

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dc.authorid0000-0002-5612-5051
dc.authorid0000-0001-5284-7439
dc.contributor.authorSahin, Caner
dc.contributor.authorMaytalman, Erkan
dc.contributor.authorSamur, Dilara Nemutlu
dc.contributor.authorDogan, Bora
dc.date.accessioned2026-01-24T12:30:53Z
dc.date.available2026-01-24T12:30:53Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractPurpose We aimed to evaluate the effects of thymoquinone and propranolol on Hep-2 cells representing laryngeal Ca cell type in comparison with cisplatin. We also evaluated their combined effects. Methods Apoptotic effects were directly analyzed via mitochondrial membrane potential and caspase-3 assays. In addition, effects on apoptosis and cell cycle via Bcl-2, Bax, P53, and Cyclin D1 mRNA expressions and effects on angiogenesis via VEGFA mRNA expression were evaluated by RT-qPCR. Results According to our results, it was determined that the anticancer effects of thymoquinone on Hep-2 cells were higher than propranolol. Our JC-1 and caspase-3 results showed an effect close to cisplatin, especially for 50 mu M thymoquinone. Significant differences were also obtained in Bcl-2, Bax, P53, and cyclin D1 results for similar concentrations compared to the control. No effect of thymoquinone was seen for VEGFA. Propranolol alone had no significant effect on JC-1 and Caspase-3. Propranolol had an effect on Bcl-2, Bax mRNA expressions compared to the control, only at 250 mu M concentration. Propranolol and its combinations increased VEGFA mRNA expression-like cisplatin. Conclusion Thymoquinone induced apoptosis and blocked the cell cycle in Hep-2 cells. The effects of propranolol, which was reported to have an antiangiogenesis effect in some studies, on apoptosis and cell cycle were limited except at high concentrations. For this cell line, why propranolol causes an increase in VEGFA expression should be evaluated extensively. Thymoquinone shows promise for cancer therapy, but studies need to be designed in vivo to evaluate the effects more reliably.
dc.description.sponsorshipAlanya Alaaddin Keyku-bat University Scientific Research Projects Unit; [2019-04-03-MAP01]
dc.description.sponsorshipThis study was supported by Alanya Alaaddin Keyku-bat University Scientific Research Projects Unit. Project ID: 2019-04-03-MAP01.
dc.identifier.doi10.1007/s00405-023-07825-0
dc.identifier.endpage2858
dc.identifier.issn0937-4477
dc.identifier.issn1434-4726
dc.identifier.issue6
dc.identifier.pmid36625867
dc.identifier.scopus2-s2.0-85145917068
dc.identifier.scopusqualityQ1
dc.identifier.startpage2849
dc.identifier.urihttps://doi.org/10.1007/s00405-023-07825-0
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5512
dc.identifier.volume280
dc.identifier.wosWOS:000911869200004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofEuropean Archives of Oto-Rhino-Laryngology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectHep-2 cell line
dc.subjectLaryngeal cancer
dc.subjectCisplatin
dc.subjectThymoquinone
dc.subjectPropranolol
dc.titleThe effect of thymoquinone and propranolol combination on epidermoid laryngeal carcinoma cell
dc.typeArticle

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