The effect of melatonin on digoxin-induced cardiac damage in cardiomyocytes

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dc.contributor.authorÖvey, İshak Suat
dc.contributor.authorÖncel, Can Ramazan
dc.date.accessioned2021-02-19T21:16:30Z
dc.date.available2021-02-19T21:16:30Z
dc.date.issued2019
dc.departmentALKÜ
dc.description.abstractOBJECTIVES: Digoxin is a cardiac glycoside which is widely used in cardiovascular medicine. Oxidative stress, as well as intracellular Ca2+ overload, plays an important role in digoxin toxicity. Transient receptor potential vanilloid 1 (TRPV1) channels are found in cardiomyocyte cells and they are activated by reactive oxygen species. We investigated the effects of digoxin toxicity and alterations in Ca2+ influx, oxidative stress and apoptosis through TRPV1 channels and modulator role of melatonin in cardiomyocytes. METHODS: The cells were divided into seven main groups as control, digoxin, digoxin+capsazepine, digoxin+melatonin, digoxin+capsazepine+melatonin, melatonin and melatonin+capsazepine groups. Cells in the groups were stimulated with capsaicin and inhibited with capsazepine in related experiments for activation and inactivation of TRPV1 channels, respectively. We measured cytosolic calcium, intracellular reactive oxygen, mitochondrial depolarization, caspase 9 and caspase 3 levels. RESULTS: The apoptosis values were significantly lower in the melatonin and digoxin+ melatonin groups than in the digoxin group of cardiomyocytes (p < 0.001). The cell viability values were higher in the digoxin+ capsazepine (p < 0.001), digoxin+melatonin (p < 0.001) and digoxin+melatonin+capsazepine (p < 0.001) groups than in the digoxin group. CONCLUSION: TRPV1 channels are overactivated during digoxin toxicity and melatonin could show a cardioprotective effect through TRPV1 channel modulation (Fig. 5, Ref. 56).
dc.identifier.doi10.4149/BLL_2019_012
dc.identifier.endpage85en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue1en_US
dc.identifier.pmid30685997
dc.identifier.scopusqualityQ2
dc.identifier.startpage78en_US
dc.identifier.urihttps://doi.org/10.4149/BLL_2019_012
dc.identifier.urihttps://hdl.handle.net/20.500.12868/452
dc.identifier.volume120en_US
dc.identifier.wosWOS:000457596700013
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherComenius Univ
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdigoxin
dc.subjectoxidative stress
dc.subjectcardiomyocyte
dc.titleThe effect of melatonin on digoxin-induced cardiac damage in cardiomyocytes
dc.typeArticle

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