Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity

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dc.contributor.authorAşçı, Halil
dc.contributor.authorÖzmen, Özlem
dc.contributor.authorErzurumlu, Yalçın
dc.contributor.authorSavaş, Hasan Basri
dc.contributor.authorTemel, Esra Nurlu
dc.contributor.authorİçten, Pelin
dc.contributor.authorHasseyid, N.
dc.date.accessioned2021-02-19T21:16:32Z
dc.date.available2021-02-19T21:16:32Z
dc.date.issued2020
dc.departmentALKÜ
dc.description.abstractWe investigated the antioxidant, anti-inflammatory and anti-apoptotic effects of pregabalin (PREG) on lipopolysaccharide (LPS) induced sepsis related cardiotoxicity via NF-k beta pathways. We used 24 female Wistar albino rats divided into three groups: control, LPS treated and LPS + PREG treated. Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), tumor necrosis factor alpha (TNF-alpha), nuclear factor kappa beta (NF-k beta)/p65, p-NF-k beta/p65, caspase-3 (Cas-3) and cleaved Cas-3 were measured in cardiac tissues and creatine kinase MB (CKMB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) levels were measured in blood samples. Also, Cas-3, granulocyte-colony stimulating factors (G-CSF), interleukin-6 (IL-6), serum amyloid A (SAA) and inducible nitric oxide synthase (iNOS) were measured immunohistochemically in heart and aorta tissue. In the LPS group; the levels of CKMB, AST, LDH, TOS, OSI increased and TAS decreased. TNF-alpha, p-NF-k beta/p65 and Cas-3 protein levels also increased in the LPS group. Immunohistochemical evaluation of the heart and aorta revealed a significant increase in the levels of Cas-3, G-CSF, SAA, IL-6 and iNOS in the LPS group. PREG treatment restored all measurements to near normal. LPS induced cardiovascular toxicity was due to inflammation, oxidative stress and apoptosis. PREG ameliorated the damage by inhibition of NF-k beta phosphorylation.
dc.identifier.doi10.1080/10520295.2020.1810315
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.pmid32921172
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1080/10520295.2020.1810315
dc.identifier.urihttps://hdl.handle.net/20.500.12868/469
dc.identifier.wosWOS:000568934100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectinflammation
dc.subjectoxidative stress
dc.subjectpregabalin
dc.subjectrats
dc.subjectsepsis
dc.titleAmeliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity
dc.typeArticle

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