Enhancing gene delivery efficiency with amphiphilic chitosan modified by myristic acid and tertiary amino groups

Basit Öğe Kaydı
dc.authorid0000-0002-3052-4556
dc.authorid0000-0002-9127-2380
dc.contributor.authorFidan, Emine Busra Eker
dc.contributor.authorBal, Kevser
dc.contributor.authorSenturk, Sema
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorDemir, Kamber
dc.contributor.authorGok, Mehmet Koray
dc.date.accessioned2026-01-24T12:31:10Z
dc.date.available2026-01-24T12:31:10Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractThe aim of this study is to synthesize new amphiphilic chitosan containing myristic acid as the hydrophobic tail and tertiary amine groups as the hydrophilic head and to evaluate the gene delivery efficiency. In this context, the primary amine groups of chitosan were first modified with myristic acid (Chi-M), followed by the modification of the methylol groups with 3-dimethylamino-1-propyl chloride hydrochloride. The chemical characterization of this chitosan formulation (Chi-MA) was determined using nuclear magnetic resonance (NMR), Fouriertransform infrared spectroscopy (FTIR) analysis and gel permeation chromatography-size exclusion chromatography. Chi-MA nanoparticles were prepared via ionic gelation, and particle size, polydispersity and zeta potential were determined. The nanoparticles were evaluated for their proton buffering capacity and gene complexing capacity. Additionally, the cytotoxicity of Chi-MA on HEK293T cells was determined via MTT assay, and the transfection efficiency of Chi-MA was analyzed by a flow cytometer. The results indicate a significant increase in gene complexing capacity (8-fold) and nanoparticle formation ability of Chi-MA compared to other chitosan formulations. Chi-MA nanoparticles showed no toxicity against HEK293T cells and exhibited the highest transfection efficiency with significantly lower nanoparticle: gene ratios compared to previous studies. These findings demonstrate the effective use of amphiphilic Chi-MA as a gene carrier.
dc.identifier.doi10.1016/j.ijbiomac.2024.136775
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid39442853
dc.identifier.scopus2-s2.0-85207092696
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.136775
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5707
dc.identifier.volume282
dc.identifier.wosWOS:001344321000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectChitosan
dc.subjectTransfection efficiency
dc.subjectGene therapy
dc.subjectMyristic acid
dc.subjectAmphiphilic polymers
dc.titleEnhancing gene delivery efficiency with amphiphilic chitosan modified by myristic acid and tertiary amino groups
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu