Effect of milk and whey on proliferation and differentiation of placental stromal cells

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dc.authorid0000-0002-5187-8839
dc.authorid0000-0002-5268-1210
dc.authorid0000-0001-8935-7724
dc.authorid0000-0001-5284-7439
dc.contributor.authorBoga, Bircan
dc.contributor.authorAkbulut, Merve
dc.contributor.authorMaytalman, Erkan
dc.contributor.authorKozanoglu, Ilknur
dc.date.accessioned2026-01-24T12:30:58Z
dc.date.available2026-01-24T12:30:58Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractFetal bovine serum (FBS), which is widely used in cell culture media, has the potential to cause medical and ethical problems. Here, an experimental study using milk or whey proteins containing essential nutrients and growth factors is presented to limit the use of FBS in cell culture media produced for cell and tissue regeneration. Study groups were formed by culturing human placenta mesenchymal stem cells, known to have high proliferation and differentiation capacity, with milk or whey solution at increasing concentrations, alone or in combination with FBS. Osteogenic and adipogenic differentiation capacities of proliferating cells were observed in FBS, milk or whey groups. Milk, whey or FBS groups obtained in P3 and after differentiation were separately analyzed for protein mRNA expression by reverse transcriptase-polymerase chain reaction (RT-qPCR). Fibroblast Growth Factor 2 (FGF2), Octamer-binding Transcription Factor 4 (OCT4), Bone Morphogenetic Protein 6 (BMP6), and adipogenic differentiation marker Peroxisome Proliferator-Activated Receptor Gamma (PPARG) were analysed by RT-qPCR. Proliferation was more pronounced in FBS alone and in its combinations with milk-whey compared to the groups in which only milk and whey were used. OCT4 mRNA and FGF2 mRNA expression decreased in differentiated cells. BMP6 mRNA expression increased with osteogenic and adipogenic stimuli. As expected, PPRG expression also increased with adipogenic stimulation. With this experimental study, evidence has been obtained that milk or whey can provide nutritional support to the culture media of repair cells and preserve the functional capacity of the cells, with a slightly more limited capacity than FBS. [GRAPHICS] .
dc.identifier.doi10.1007/s10616-023-00585-z
dc.identifier.issn0920-9069
dc.identifier.issn1573-0778
dc.identifier.pmid37655272
dc.identifier.scopus2-s2.0-85164963008
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1007/s10616-023-00585-z
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5535
dc.identifier.wosWOS:001032505900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCytotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectMesenchymal stem cell
dc.subjectMilk
dc.subjectWhey
dc.subjectProliferation
dc.subjectDifferentiation
dc.titleEffect of milk and whey on proliferation and differentiation of placental stromal cells
dc.typeArticle

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