Particle stabilised high internal phase emulsion scaffolds with interconnected porosity facilitate cell migration

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dc.authorid0000-0002-2321-1367
dc.authorid0000-0002-1030-939X
dc.authorid0000-0003-1456-1071
dc.authorid0000-0002-2224-7325
dc.authorid0000-0002-8612-5001
dc.contributor.authorMunive-Olarte, Areli
dc.contributor.authorDurgut, Enes
dc.contributor.authorVerbruggen, Stefaan W.
dc.contributor.authorClaeyssens, Frederik
dc.contributor.authorReilly, Gwendolen C.
dc.date.accessioned2026-01-24T12:31:30Z
dc.date.available2026-01-24T12:31:30Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractA key challenge in bone tissue engineering (BTE) is designing structurally supportive scaffolds, mimicking the native bone matrix, yet also highly porous to allow nutrient diffusion, cell infiltration, and proliferation. This study investigated the effect of scaffold interconnectivity on human bone marrow stromal cell (BMSC) behaviour. Highly interconnected, porous scaffolds (polyHIPEs) were fabricated using the emulsion templating method from 2-ethylhexyl acrylate/isobornyl acrylate (IBOA) and stabilised with similar to 200 nm IBOA particles. Pore interconnectivity was tuned by varying the internal phase fraction from 75%-85% and characterised by the degree of openness, Euler number, frequency, and size of pore interconnects. The attachment, proliferation, infiltration, and osteogenic differentiation of the BMSC cell line (Y201) were evaluated on these scaffolds. Results showed that high pore interconnectivity facilitated diffusion and cell infiltration throughout the scaffolds. Furthermore, the most interconnected scaffolds enhanced osteogenic differentiation of Y201 cells, as evidenced by elevated alkaline phosphatase activity and increased calcium and collagen production compared to less interconnected scaffolds. These findings emphasise the importance of scaffold interconnectivity in BTE for efficient nutrient transport, facilitating cell migration and infiltration, and supporting the development of interconnected cell networks that positively influence osteogenic differentiation.
dc.description.sponsorshipConsejo Nacional de, Ciencias y Tecnologas (CONACYT)http://dx.doi.org/10.13039/501100003141 [795349]; NationalCouncil of Science and Technology (CONACYT) [Y201]
dc.description.sponsorshipAM-O acknowledges the scholarship with number 795349 granted by the NationalCouncil of Science and Technology (CONACYT, now SECIHTI). Authors thank Professor Paul Genever (University of York) for supplying the BMSC clonal line Y201.
dc.identifier.doi10.1088/1748-605X/ae05de
dc.identifier.issn1748-6041
dc.identifier.issn1748-605X
dc.identifier.issue6
dc.identifier.pmid40930136
dc.identifier.scopus2-s2.0-105017881035
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1088/1748-605X/ae05de
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5931
dc.identifier.volume20
dc.identifier.wosWOS:001587816800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherIop Publishing Ltd
dc.relation.ispartofBiomedical Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectbone scaffold
dc.subjectpore interconnectivity
dc.subjectcell infiltration
dc.subjectosteogenic differentiation
dc.subjecttissue engineering
dc.titleParticle stabilised high internal phase emulsion scaffolds with interconnected porosity facilitate cell migration
dc.typeArticle

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