Adrenergic receptor behaviors of mesenchymal stem cells obtained from different tissue sources and the effect of the receptor blockade on differentiation

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dc.authorid0000-0003-3585-2599
dc.authorid0000-0002-5268-1210
dc.authorid0000-0001-5284-7439
dc.contributor.authorMaytalman, Erkan
dc.contributor.authorAlizadeh Yegani, Arash
dc.contributor.authorKozanoglu, Ilknur
dc.contributor.authorAksu, Fazilet
dc.date.accessioned2026-01-24T12:31:27Z
dc.date.available2026-01-24T12:31:27Z
dc.date.issued2022
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractIn this study, it was aimed to analyze behavioral changes of adrenergic receptors (ARs) in first three passages and osteogenic/adipogenic differentiation of mesenchymal stem cells (MSCs) derived from placenta fetal membrane (FM) and bone marrow (BM). It was also aimed to evaluate effects of receptor blockade on differentiation. We obtained first three passages of MSCs from placenta and BM samples. For cell identification, the cells were analyzed by flow cytometry using CD34, CD45 and CD3, CD105 antibodies in each passage. The effects of propranolol and phenoxybenzamine at incremental doses were analyzed by MTT. In addition, cell cultures were separately maintained with the blockers or without after second passage. After each passage and differentiation, alpha 1A, alpha 1B, alpha 2A, alpha 2B, beta 1, beta 2, beta 3 AR-mRNA expressions analyzed by RT-qPCR technique. BMP6 and PPARG mRNA expressions only after differentiation and passage 3 were analyzed. A microscopic examination was also performed. Our results showed that AR expression behaviors were different in MSCs obtained from different tissue sources. In particular, alpha(1A)-AR and alpha(2A)-AR were expressed with considerably high coefficients in differentiation under blocker effect in BM-derived MSCs. No such coefficients were observed in any group of placental MSCs. In addition, it was found that the blockers stimulated adipogenesis in BM-derived MSCs during osteogenic differentiation. MSCs exhibit protein expressions that vary according to source of tissue and differentiation. Given that MSCs from different sources are used for repair and modulation, our study makes implications of this variable expression intriguing in the clinical practice.
dc.description.sponsorshipCukurova University Research Fund [TF2013D1]
dc.description.sponsorshipThis work was supported by the Cukurova University Research Fund [Project No.: TF2013D1].
dc.identifier.doi10.1080/10799893.2021.1957931
dc.identifier.endpage360
dc.identifier.issn1079-9893
dc.identifier.issn1532-4281
dc.identifier.issue4
dc.identifier.pmid34323168
dc.identifier.scopus2-s2.0-85111632822
dc.identifier.scopusqualityQ3
dc.identifier.startpage349
dc.identifier.urihttps://doi.org/10.1080/10799893.2021.1957931
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5891
dc.identifier.volume42
dc.identifier.wosWOS:000679329300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Receptors and Signal Transduction
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectMesenchymal stem cells
dc.subjectadrenergic receptors
dc.subjectdifferentiation
dc.titleAdrenergic receptor behaviors of mesenchymal stem cells obtained from different tissue sources and the effect of the receptor blockade on differentiation
dc.typeArticle

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