Vanillic acid induces mitochondrial biogenesis in SH-SY5Y cells

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dc.authorid0000-0003-1179-593X
dc.contributor.authorAy, Muhammet
dc.date.accessioned2026-01-24T12:30:58Z
dc.date.available2026-01-24T12:30:58Z
dc.date.issued2022
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractBackground Mitochondrial dysfunction has been recognized as an important mechanism of neurodegeneration. Accumulating evidence now suggests that defects in mitochondrial biogenesis can cause mitochondrial dysfunction in neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Therefore, identifying small molecules that can stimulate mitochondrial biogenesis may represent a therapeutic strategy for neuroprotection. The aim of this study was to investigate the effects of a natural compound vanillic acid (VA) on mitochondrial biogenesis and the expression of PD-related genes in SH-SY5Y cells. Methods and Results After determining the IC50 and non-toxic concentrations of VA, SH-SY5Y cells were exposed to a non-toxic dose of VA (300 mu M) for 18 h. VA treatment resulted in significant increases in the mRNA expressions of mitochondrial biogenesis markers, PGC-1 alpha and TFAM. Moreover, treatment of SH-SY5Y cells with 300 mu M VA for 24 h significantly elevated the mitochondrial DNA (mtDNA) copy number and mitochondrial mass. Furthermore, the effects of VA on the expression of PD-related genes were analyzed using a real-time PCR array. The PCR array analysis revealed that VA can induce the expression of some genes involved in neuronal differentiation and also affect the expression of two PARK genes, PARK2 and LRRK2, whose mutations cause familial PD. Conclusions Together, these findings indicate that VA could serve as a potential neuroprotective agent by virtue of its ability to stimulate mitochondrial biogenesis in neuronal cells and to alter the expression of some genes related to the pathogenesis of PD and neuronal differentiation.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [118S548]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Project No: 118S548.
dc.identifier.doi10.1007/s11033-022-07284-6
dc.identifier.endpage4449
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue6
dc.identifier.pmid35249168
dc.identifier.scopus2-s2.0-85125618981
dc.identifier.scopusqualityQ3
dc.identifier.startpage4443
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07284-6
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5552
dc.identifier.volume49
dc.identifier.wosWOS:000765190200002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectVanillic acid
dc.subjectMitochondrial biogenesis
dc.subjectParkinson's disease
dc.subjectSH-SY5Y
dc.titleVanillic acid induces mitochondrial biogenesis in SH-SY5Y cells
dc.typeArticle

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