Protective effects of N-acetyl cysteine against paclitaxel-induced cardiotoxicity through modulation of transient receptor potential melastatin 2 channels

Basit Öğe Kaydı
dc.contributor.authorÖncel, Can Ramazan
dc.contributor.authorÖvey, İshak Suat
dc.date.accessioned2021-02-19T21:16:31Z
dc.date.available2021-02-19T21:16:31Z
dc.date.issued2019
dc.departmentALKÜ
dc.description.abstractAim : In our study, we investigated the paclitaxel induced cardiotoxicity and alterations in Ca2+ influx, oxidative stress and apoptosis through transient receptor potential melastatin 2 (TRPM2) channels and modulator role of N-acetyl cysteine (NAC) in cardiomyocytes. Material and Methods : All cells were cultured at 37 degrees C. The cells were divided into seven main groups. Cells in the paclitaxel group were incubated with 2.5 mu M Paclitaxel for 12 hours and cells in the NAC+Paclitaxel group were incubated with 2.5 mu M Paclitaxel for 12 hours and then incubated with 10 mu M NAC for 24 hours. Intracellular free calcium concentration, reactive oxygen species (ROS) production measurements and cell viability analyses were done according to the study protocol. Results : Cytosolic calcium levels, apoptosis levels, intracellular ROS production levels were lower in paclitaxel+NAC group than in the paclitaxel group of cardiomyocytes. Also values were markedly lower in the paclitaxel+NAC+antranilic acid group when compared to the paclitaxel+ NAC group. Conclusion : We found that TRPM2 channels are overactivated during paclitaxel induced cardiotoxicity and NAC could show a cardioprotective effect through TRPM2 channel modulation.
dc.identifier.doi10.12996/gmj.2019.39
dc.identifier.endpage161en_US
dc.identifier.issn2147-2092
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ4
dc.identifier.startpage155en_US
dc.identifier.urihttps://doi.org/10.12996/gmj.2019.39
dc.identifier.urihttps://hdl.handle.net/20.500.12868/458
dc.identifier.volume30en_US
dc.identifier.wosWOS:000462182300009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherGazi Univ, Fac Med
dc.relation.ispartofGazi Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectpaclitaxel
dc.subjectcardiomyocyte
dc.subjecttransient receptor potential melastatin 2
dc.subjectN-acetyl cysteine
dc.titleProtective effects of N-acetyl cysteine against paclitaxel-induced cardiotoxicity through modulation of transient receptor potential melastatin 2 channels
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu