Enhancing transfection efficiency of primary cell lines using different terminated PBAE structures without endcapping reaction

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dc.authorid0000-0002-9127-2380
dc.authorid0000-0003-2185-2619
dc.contributor.authorDemir, Irmak
dc.contributor.authorCelik, Sibel Kucukertugrul
dc.contributor.authorBal, Kevser
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorSenturk, Sema
dc.contributor.authorDemir, Kamber
dc.contributor.authorGok, Mehmet Koray
dc.date.accessioned2026-01-24T12:30:53Z
dc.date.available2026-01-24T12:30:53Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractGene therapy holds promise for a wide range of diseases, including Alzheimer's, diabetes, and cancer, and requires the efficient transfer of nucleic acids into cells. However, transfection in primary cells is still problematic and requires the development of new transfection agents. Poly (beta-amino ester) (PBAE) has attracted great attention in transfection research due to their low toxicity, high gene loading capacity, endosomal escape ability, and biodegradability properties. In this study, two new PBAEs with different molecular weights are synthesized that could provide high viability and transfection efficiency in primary cells. They are characterized using FTIR and 1H NMR analysis. GPC-SEC system is also used to calculate the average molecular weight (Mw) and polydispersity index. PBAE nanoparticle preparation is carried out using the nanoprecipitation technique. The gene loading capacity, protective ability against nuclease degradation, and proton buffering capacity of nanoparticles are determined. Additionally, the morphology of PBAEA:pEGFN1 complexes was investigated by STEM analysis. Finally, their cytotoxicity and transfection efficiency in primary ovine fibroblast (POF) cells are also investigated. The results reveal that the new PBAE with higher Mw achieves quite high transfection efficiency of about 87% and did not show any cytotoxic effects on these cells. These findings suggest that PBAE is a promising option to achieve high transfection efficiency in primary cells.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye [1919B012002302/2209-A]; Presidency of Turkey, Presidency of Strategy and Budget [DPT-2019 K12-149071]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkiye (1919B012002302/2209-A) and Presidency of Turkey, Presidency of Strategy and Budget (DPT-2019 K12-149071).
dc.identifier.doi10.1007/s00396-025-05449-w
dc.identifier.endpage1719
dc.identifier.issn0303-402X
dc.identifier.issn1435-1536
dc.identifier.issue8
dc.identifier.scopus2-s2.0-105007081525
dc.identifier.scopusqualityQ2
dc.identifier.startpage1705
dc.identifier.urihttps://doi.org/10.1007/s00396-025-05449-w
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5510
dc.identifier.volume303
dc.identifier.wosWOS:001500323800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofColloid and Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectPoly (beta-amino ester)
dc.subjectPrimary ovine fibroblast
dc.subjectTransfection
dc.subjectGene therapy
dc.titleEnhancing transfection efficiency of primary cell lines using different terminated PBAE structures without endcapping reaction
dc.typeArticle

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