Silane and amino acid functionalized PBAEs for enhanced gene Delivery: Synthesis, characterization, and transfection efficiency

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dc.authorid0000-0002-9127-2380
dc.authorid0000-0002-3052-4556
dc.authorid0000-0003-2497-9359
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorBal, Kevser
dc.contributor.authorSenturk, Sema
dc.contributor.authorDemir, Kamber
dc.contributor.authorGok, Mehmet Koray
dc.date.accessioned2026-01-24T12:31:12Z
dc.date.available2026-01-24T12:31:12Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractThis study aimed to synthesize, characterize, and evaluate the transfection efficiency of end group-modified branched poly (R-amino ester) (PBAE) based polymers containing silane groups to be used as gene carriers. Branched PBAE polymers were synthesized using bisphenol A ethoxylate diacrylate, diethylenetriamine, and N-[3-(trimethoxysilyl)propyl] ethylenediamine, and various amino acids (histidine, isoleucine, serine, methionine, phenylalanine, arginine) were added for end group modification. The structures of the polymers were confirmed by nuclear magnetic resonance (NMR) and Fourier-transform infrared spectroscopy (FTIR) analysis. Nano-particles were prepared using the nanoprecipitation technique, and their plasmid DNA complexing and protection capacity and endosomal escape abilities were determined. Finally, the cytotoxicity of the nanoparticles on HeLa and HEK293T cell lines was tested by the MTT test, and their transfection efficiency was tested by flow cytometry and fluorescence microscopy. All the tested nanoparticles showed lower toxicity in HeLa cells than in HEK293T cells. Transfection efficiency followed an increasing order, starting from the linear PBAE, progressing to silane modification, and subsequently with modifications by histidine, isoleucine, serine, methionine, phenylalanine, and arginine. This study highlights the potential of silane and amino acid-functionalized PBAE polymers as gene carriers and the impact of these modifications on transfection efficiency.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [BIDEB-2218, 121C448, 2024/014909, PCT-TR2024-051675]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Tuerkiye (TUBITAK) BIDEB-2218 Domestic Post-doctoral Research Fellowship, grant number 121C448. We would like to acknowledge that the nanoparticulate systems produced in this study have been submitted for a patent application to the Turkish Patent and Trademark Office (Application No: 2024/014909) , and a Patent Cooperation Treaty (PCT) application has been filed under PCT-TR2024-051675.
dc.identifier.doi10.1016/j.jddst.2025.106990
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-105003943670
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2025.106990
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5728
dc.identifier.volume109
dc.identifier.wosWOS:001486309500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectGene delivery
dc.subjectPoly (beta-amino ester) (PBAE)
dc.subjectAmino acid end-group modification
dc.titleSilane and amino acid functionalized PBAEs for enhanced gene Delivery: Synthesis, characterization, and transfection efficiency
dc.typeArticle

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