Microbial transformation of cycloastragenol and astragenol by endophytic fungi isolated from astragalus species

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dc.contributor.authorEkiz, Güner
dc.contributor.authorYılmaz, Sinem
dc.contributor.authorYusufoğlu, Hasan
dc.contributor.authorKırmızıbayrak, Petek Ballar
dc.contributor.authorBedir, Erdal
dc.date.accessioned2021-02-19T21:20:50Z
dc.date.available2021-02-19T21:20:50Z
dc.date.issued2019
dc.departmentALKÜ
dc.description.abstractBiotransformation of Astragalus sapogenins (cycloastragenol (1) and astragenol (2)) by Astragalus species originated endophytic fungi resulted in the production of five new metabolites (3, 7, 10, 12, 14) together with 10 known compounds. The structures of the new compounds were established by NMR spectroscopic and HRMS analysis. Oxygenation, oxidation, epoxidation, dehydrogenation, and ring cleavage reactions were observed on the cycloartane (9,19-cyclolanostane) nucleus. The ability of the compounds to increase telomerase activity in neonatal cells was also evaluated. After prescreening studies to define potent telomerase activators, four compounds were selected for subsequent bioassays. These were performed using very low doses ranging from 0.1 to 30 nM compared to the control cells treated with DMSO. The positive control cycloastragenol and 8 were found to be the most active compounds, with 5.2- (2 nM) and 5.1- (0.5 nM) fold activations versus DMSO, respectively. At the lowest dose of 0.1 nM, compounds 4 and 13 provided 3.5- and 3.8-fold activations, respectively, while cycloastragenol showed a limited activation (1.5-fold). © 2019 American Chemical Society and American Society of Pharmacognosy.
dc.description.sponsorship114Z958 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBİTAK
dc.description.sponsorshipThis project was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Project No. 114Z958). We are very grateful to Bionorm Natural Products for donating cycloastragenol and astragenol, and special thanks are due to the NMR spectrometer operator, A. Anwarulhaque, of Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia.
dc.identifier.doi10.1021/acs.jnatprod.9b00336
dc.identifier.endpage2985en_US
dc.identifier.issn0163-3864
dc.identifier.issue11en_US
dc.identifier.pmid31713424
dc.identifier.scopusqualityQ1
dc.identifier.startpage2979en_US
dc.identifier.urihttps://doi.org/10.1021/acs.jnatprod.9b00336
dc.identifier.urihttps://hdl.handle.net/20.500.12868/722
dc.identifier.volume82en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.ispartofJournal of Natural Products
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleMicrobial transformation of cycloastragenol and astragenol by endophytic fungi isolated from astragalus species
dc.typeArticle

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