Potent telomerase activators from a novel sapogenin via biotransformation utilizing Camarosporium laburnicola, an endophytic fungus

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dc.contributor.authorKüçüksolak, Melis
dc.contributor.authorYilmaz, Sinem
dc.contributor.authorBallar Kirmizibayrak, Petek
dc.contributor.authorBedir, Erdal
dc.date.accessioned2026-01-24T12:20:49Z
dc.date.available2026-01-24T12:20:49Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractBackground: Cycloartane-type triterpenoids possess important biological activities, including immunostimulant, wound healing, and telomerase activation. Biotransformation is one of the derivatization strategies of natural products to improve their bioactivities. Endophytic fungi have attracted attention in biotransformation studies because of their ability to perform modifications in complex structures with a high degree of stereospecificity. Results: This study focuses on biotransformation studies on cyclocephagenol (1), a novel cycloartane-type sapogenin from Astragalus species, and its 12-hydroxy derivatives (2 and 3) to obtain new telomerase activators. Since the hTERT protein levels of cyclocephagenol (1) and its 12-hydroxy derivatives (2 and 3) on HEKn cells were found to be notable, biotransformation studies were carried out on cyclocephagenol and its 12-hydroxy derivatives using Camarosporium laburnicola, an endophytic fungus isolated from Astragalus angustifolius. Later, immunoblotting and PCR-based ELISA assay were used to screen starting compounds and biotransformation products for their effects on hTERT protein levels and telomerase activation. All compounds showed improved telomerase activation compared to the control group. Conclusions: As a result of biotransformation studies, seven new metabolites were obtained and characterized, verifying the potential of C. laburnicola as a biocatalyst. Additionally, the bioactivity results showed that this endophytic biocatalyst is unique in transforming the metabolites of its host to afford potent telomerase activators. © 2023, The Author(s).
dc.description.sponsorshipEge Üniversitesi; Çankiri Karatekin Üniversitesi
dc.identifier.doi10.1186/s12934-023-02069-3
dc.identifier.issn1475-2859
dc.identifier.issue1
dc.identifier.pmid37024895
dc.identifier.scopus2-s2.0-85151812060
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s12934-023-02069-3
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4584
dc.identifier.volume22
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBioMed Central Ltd
dc.relation.ispartofMicrobial Cell Factories
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20260121
dc.subjectAnti-aging
dc.subjectBiotransformation
dc.subjectEndophytic Fungi
dc.subjectSapogenin
dc.subjectTelomerase activator
dc.titlePotent telomerase activators from a novel sapogenin via biotransformation utilizing Camarosporium laburnicola, an endophytic fungus
dc.typeArticle

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