Development of recombinant protein-based nanoparticle systems for inducing tumor cell apoptosis: In vitro evaluation of their cytotoxic and apoptotic effects on cancer cells

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dc.authorid0000-0002-1793-0859
dc.authorid0000-0002-3052-4556
dc.authorid0000-0001-8473-5896
dc.authorid0000-0003-2497-9359
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorGok, Mehmet Koray
dc.contributor.authorPekmez, Murat
dc.contributor.authorTayhan, Secil Erden
dc.contributor.authorOzguemues, Saadet
dc.contributor.authorGokce, Isa
dc.contributor.authorArda, Nazli
dc.date.accessioned2026-01-24T12:31:12Z
dc.date.available2026-01-24T12:31:12Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractMyeloid leukemia cell differentiation protein (Mcl-1) is a B -cell lymphoma 2 (Bcl-2) family antiapoptotic protein that regulates cellular apoptosis. Mcl-1 expression is important for cancer cell survival and a prospective target in cancer treatment. Bcl-2 homology 3 (BH3) mimetic peptides, such as the Mcl-1-specific MS1 peptide that targets conserved BH3 domains, are highly specific Mcl-1 inhibitors. However, the application of these peptides is limited due to their metabolic instability, degradation by cellular proteases, and low cell permeability. In this study, it was aimed to form nanostructures to increase the cellular uptake and stability of the Mcl-1 inhibitor MS1 peptide. The Bim BH3 mimetic MS1 peptide was produced recombinantly in fusion with mNeonGreen fluorescent protein and polyhistidine (6xHisTag). Self -assembled protein nanoparticles were fabricated from the recombinant MS1-mNeonGreen protein. Furthermore, the MS1-mNeonGreen protein was encapsulated into polymeric nanoparticles. The cytotoxic effects of these nanoparticles on cancer cells [cervical cancer cell line (HeLa), lung cancer cell line (A549) and breast cancer cell line (MDA-MB-231)] and healthy cell line [Human umbilical vein endothelial cells (HUVEC)] was analyzed by MTT test. The apoptotic effect of nanoparticles on these cells was investigated by determining the antiapoptotic and proapoptotic protein levels. As a result, it was revealed that the nanoparticles have a specific cytotoxic effect against the cells and induce apoptosis.
dc.description.sponsorshipResearch Council of Turkey, Turkey (TUBITAK) [-BIDEB 2211/A-Domes-tic PhD]; TUBITAK; Istanbul University [PCT/TR2022/051 620]
dc.description.sponsorshipThis study was funded by Scientific Research Projects Coordination Unit of Istanbul University, Turkey (Project number: FBA-2019-34211) . This study was partially produced from ozlem KAPLAN Ph.D. thesis. Ozlem KAPLAN was supported by The Scientific and Technological Research Council of Turkey, Turkey (TUBITAK) -BIDEB 2211/A-Domestic PhD Scholarship Program. Authors thank TUBITAK and Istanbul University for their supports. A patent application has been made for the nanoparticulate systems obtained in this study (PCT/TR2022/051 620) .r Research Council of Turkey, Turkey (TUBITAK) -BIDEB 2211/A-Domes-tic PhD Scholarship Program. Authors thank TUBITAK and Istanbul University for their supports. A patent application has been made for the nanoparticulate systems obtained in this study (PCT/TR2022/051 620) .
dc.identifier.doi10.1016/j.jddst.2024.105565
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85188120069
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2024.105565
dc.identifier.urihttps://hdl.handle.net/20.500.12868/5726
dc.identifier.volume95
dc.identifier.wosWOS:001218017400001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260121
dc.subjectMcl-1 inhibition
dc.subjectCancer
dc.subjectRecombinant protein
dc.subjectProtein -based nanoparticles
dc.subjectHybrid polymeric nanoparticles
dc.subjectMS1 peptide
dc.titleDevelopment of recombinant protein-based nanoparticle systems for inducing tumor cell apoptosis: In vitro evaluation of their cytotoxic and apoptotic effects on cancer cells
dc.typeArticle

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