EFFECT OF MELATONIN AND CISPLATIN ON CERVICAL CANCER CELLS: ROLE OF TRPV1 CHANNELS

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dc.contributor.authorOvey, Ishak Suat
dc.contributor.authorAtes, Sezin
dc.date.accessioned2026-01-24T12:26:49Z
dc.date.available2026-01-24T12:26:49Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractCisplatin, a widely used treatment for cervical cancer, and melatonin, known for enhancing the apoptotic effects of cisplatin, both play crucial roles in our study. We investigated the combined effects of these two agents on HeLa cervical cancer cells, with focus on the role of TRPV1 channels, which we found to be of significant importance. According to the treatment performed, the cell samples were divided into seven main groups: control, cisplatin, cisplatin + capsazepine (TRPV1 antagonist), melatonin, melatonin + capsazepine, cisplatin + melatonin, and cisplatin + melatonin + capsazepine. Each group was stimulated with capsaicin (TRPV1 agonist) in all analyses. We measured apoptosis, cytosolic calcium, intracellular reactive oxygen species, mitochondrial depolarization, caspase-9, and caspase-3 levels. The combined application of cisplatin and melatonin led to a significant increase in apoptosis levels, which were statistically significantly higher compared to both the control group and the group treated with cisplatin alone (p < 0.001) (Fig. 2). This notable increase underscores the potential of this combined treatment in treating cervical cancer. Our findings strongly suggest that melatonin could be a promising adjunct therapy for cisplatin-induced cervical cancer. By elevating cytosolic Ca2+ levels, promoting apoptosis, and increasing intracellular ROS levels through TRPV1 channels, melatonin indicated potential as an effective
dc.identifier.doi10.7546/CRABS.2025.10.10
dc.identifier.endpage1515
dc.identifier.issn1310-1331
dc.identifier.issue10
dc.identifier.scopus2-s2.0-105019943315
dc.identifier.scopusqualityQ4
dc.identifier.startpage1506
dc.identifier.urihttps://doi.org/10.7546/CRABS.2025.10.10
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4954
dc.identifier.volume78
dc.identifier.wosWOS:001606212700010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPubl House Bulgarian Acad Sci
dc.relation.ispartofComptes Rendus De L Academie Bulgare Des Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectcervix cancer
dc.subjectTRPV1 channel
dc.subjectcisplatin
dc.subjectmelatonin
dc.subjectoxidative stress
dc.titleEFFECT OF MELATONIN AND CISPLATIN ON CERVICAL CANCER CELLS: ROLE OF TRPV1 CHANNELS
dc.typeArticle

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