Antibacterial activity of silver nanoparticles activated by photodynamic therapy in infected root canals

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dc.contributor.authorAydın, Hakan
dc.contributor.authorEr, Kürşat
dc.contributor.authorKuştarcı, Alper
dc.contributor.authorAkarsu, Murat
dc.contributor.authorGençer, Gül Merve
dc.contributor.authorEr, Hakan
dc.contributor.authorFelek, Rasih
dc.date.accessioned2021-02-19T21:20:45Z
dc.date.available2021-02-19T21:20:45Z
dc.date.issued2020
dc.departmentALKÜ
dc.description.abstractBACKGROUND: Root canal disinfection includes mechanical, chemical and biological struggle against microorganisms (MOs). Photodynamic therapy (PDT) and nanoparticle (NP) agents may be proposed as an alternative for use against intracanal infections due to their ability to disrupt biofilm and prevent bacterial adhesion to dentin. The use of NP agents in combination with light/photosensitizer (PS) agents increases the efficiency of PDT in root canal disinfection. OBJECTIVES: The aim of the study was to evaluate the effect of light application - PDT - on the antibacterial activity of the combination of a PS agent (toluidine blue O -TBO) and an NP agent (silver nanoparticles - AgNPs) for the disinfection of the root canals inoculated with Enterococcus faecalis (E. faecalis). MATERIAL AND METHODS: In this study, concentrations of 20 ppm of TBO and 10 ppm of AgNPs, which showed the highest antibacterial activity against E. faecalis in the TBO/AgNPs combination, were used according to the preliminary studies. After instrumentation, 120 human, single-rooted, straight-canal mandibular premolars of a standard length of 13 mm were contaminated with bacteria, and experimental procedures were conducted against 21-day-old mature biofilm. The teeth were randomly divided into 5 main experimental groups: TBO/light; AgNPs; TBO/AgNPs; AgNPs/light; and TBO/AgNPs/light. Then, these main groups were divided into 2 subgroups each, according to the 2 application time periods (30 s and 60 s) (n = 10). The remaining 20 teeth constituted positive and negative control groups. The data was analyzed with the Kolmogorov-Smirnov test, one-way analysis of variance (ANOVA), Tukey's honestly significant difference (HSD) test, and the Bonferroni correction. RESULTS: The NaOCl group provided a bacterial reduction that was higher than in all other groups in a statistically significant manner. Light application on the TBO/AgNPs combination was the group that provided the highest bacterial reduction after NaOCl. CONCLUSIONS: The photoactivation of the TBO/AgNPs combination led to an increase in the effect of PDT, and it has the potential to be used as an adjunct for disinfection of the root canal system.
dc.identifier.doi10.17219/dmp/123615
dc.identifier.endpage400en_US
dc.identifier.issn2300-9020
dc.identifier.issue4en_US
dc.identifier.pmid33444488
dc.identifier.scopusqualityQ2
dc.identifier.startpage393en_US
dc.identifier.urihttps://doi.org/10.17219/dmp/123615
dc.identifier.urihttps://hdl.handle.net/20.500.12868/651
dc.identifier.volume57en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor0-belirlenecek
dc.language.isoen
dc.publisherNLM (Medline)
dc.relation.ispartofDental and medical problems
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectphotodynamic therapy
dc.subjectroot canal disinfection
dc.subjectsilver nanoparticles
dc.titleAntibacterial activity of silver nanoparticles activated by photodynamic therapy in infected root canals
dc.typeArticle

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