Apoptosis Induction Through Increased TRPV1 Activation by Synergic Effect of Melatonin and Doxorubicin in Human Osteosarcoma and Chondrosarcoma Cell Lines

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dc.contributor.authorKocak, Ahmet
dc.contributor.authorGülcü, Anıl
dc.contributor.authorÖvey, İshak Suat
dc.date.accessioned2026-01-24T12:00:50Z
dc.date.available2026-01-24T12:00:50Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractAim: We aimed to reveal the role of doxorubicin (Dox), melatonin (Mel) and transient receptor potential Vanilloid 1 (TRPV1) channels in bone and cartilage cancer cells during the treatment process. Human Bone Osteosarcoma (Saos-2/An1) and Human Chondrosarcoma (Hs 819.T) cell lines were used to prepare in-vitro experiment models. Methods: Both cell lines were cultured at 37°C. We have separated each cell line into five groups as follows: Controls, Dox, Dox+Capsazepine (Cpz), Dox+Melatonin (Mel), and combined Dox+Mel+Cpz given group. Capsaicin and capsazepine were added to cell culture mediums to activate or inactivate the TRPV1 channels, respectively. Cytosolic calcium, apoptosis, intracellular reactive oxygen, mitochondrial depolarization, caspase-3 and caspase-9 levels were measured. Results: Increased apoptotic activity was detected in doxorubicin given cell lines (Group II) when compared with the controls (p?0.001). There was also a significantly higher apoptotic level in Dox+Mel group (Group IV), when compared with only Dox given group (p?0.001). TRPV1 inhibition applied groups (Group III and V) have had lower apoptotic levels than other drug administered groups (p?0.001). Conclusion: This study has indicated that apoptotic effects of Dox and Mel on both osteosarcoma and chondrosarcoma were strictly associated to TRPV1 channels, and that TRPV1 channels played an important role in whole mitochondria dependent pathways of apoptosis, which in turn may lead to increased intracellular Ca+2 levels and mitochondrial depolarization.
dc.identifier.doi10.30565/medalanya.1313745
dc.identifier.endpage152
dc.identifier.issn2587-0319
dc.identifier.issue2
dc.identifier.startpage145
dc.identifier.trdizinid1212343
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1212343
dc.identifier.urihttps://doi.org/10.30565/medalanya.1313745
dc.identifier.urihttps://hdl.handle.net/20.500.12868/3739
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofActa Medica Alanya
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260121
dc.subjectMelatonin
dc.subjectchondrosarcoma
dc.subjectOsteosarcoma
dc.subjectdoxorubicin
dc.subjectTRPV1
dc.titleApoptosis Induction Through Increased TRPV1 Activation by Synergic Effect of Melatonin and Doxorubicin in Human Osteosarcoma and Chondrosarcoma Cell Lines
dc.typeArticle

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