Effects of Collagen Coating, Fetal Bovine Serum Concentration, Differentiation Agents, and Neurotoxin Application on In Vitro Modeling of Parkinson's Disease Using SH-SY5Y Cell Culture

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dc.contributor.authorKoçancı, Fatma Gonca
dc.date.accessioned2026-01-24T12:00:50Z
dc.date.available2026-01-24T12:00:50Z
dc.date.issued2024
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractAim: This study aims to optimize SH-SY5Y culture conditions to develop precise in vitro disease models for Parkinson's disease (PD) research. It seeks to investigate the effects of various factors such as collagen coating, fetal bovine serum concentration, differentiation agents, and neurotoxin treatments on cellular behavior and disease modeling. Materials and Methods: The human neuroblastoma SH-SY5Y cell line was cultured in DMEM/F12 supplemented with heat-inactivated fetal bovine serum (FBS), penicillin-streptomycin, and L-glutamine. Collagen coating was applied to assess its impact on cell differentiation, while the ideal cell density and serum ratio for generating neurite-like cells were determined through experimentation. The MTT assay was employed to evaluate the cytotoxic effects of paraquat, while dopamine levels were quantified using ELISA. Gene expression was analyzed via real-time qPCR. Immunofluorescence staining and neurite length measurements were conducted to validate the PD model and assess cellular morphology. Results: Cells cultured at a density of 5x103 cells/cm2 with collagen and 2% FBS exhibited characteristics of dopaminergic neurons upon exposure to retinoic acid. Conversely, paraquat treatment induced neurotoxicity, resulting in decreased dopamine levels and impaired neurite outgrowth. Conclusion: This study investigated the optimization of SH-SY5Y cell culture conditions for PD modeling. Key findings include optimal cell density, FBS concentration, and beneficial effects of collagen coating. Additionally, an effective paraquat neurotoxicity protocol has been established, providing a solid framework for future research on neuronal differentiation and degeneration.
dc.identifier.doi10.30565/medalanya.1459470
dc.identifier.endpage109
dc.identifier.issn2587-0319
dc.identifier.issue2
dc.identifier.startpage105
dc.identifier.trdizinid1262483
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1262483
dc.identifier.urihttps://doi.org/10.30565/medalanya.1459470
dc.identifier.urihttps://hdl.handle.net/20.500.12868/3751
dc.identifier.volume8
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofActa Medica Alanya
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260121
dc.subjectParkinson
dc.subjectSH-SY5Y cells
dc.subjectneurotoxicity
dc.subjectneuronal differentiation
dc.subjectin vitro modeling
dc.titleEffects of Collagen Coating, Fetal Bovine Serum Concentration, Differentiation Agents, and Neurotoxin Application on In Vitro Modeling of Parkinson's Disease Using SH-SY5Y Cell Culture
dc.typeArticle

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