Investigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line

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dc.contributor.authorImamoglu, Rızvan
dc.contributor.authorKaplan, Ozlem
dc.contributor.authorGök, Mehmet Koray
dc.contributor.authorGokce, Isa
dc.date.accessioned2026-01-24T12:00:53Z
dc.date.available2026-01-24T12:00:53Z
dc.date.issued2025
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractGenome editing technology is a promising, popular technology for diagnosing and treating many diseases. One of the most popular techniques utilized in genome editing research nowadays is the CRISPR/Cas system. The CRISPR/Cas system holds considerable potential for diagnostic and therapeutic applications due to its ease of use, speed, and affordability. The safe and effective delivery of the CRISPR-based genome editing system to the intended human body cells is one of the system's problems. This study investigated the silencing of the COX-2 gene via the CRISPR/Cas system using a P?AE-based transfection agent in the HUVEC cell line. Western blot analysis was performed to confirm COX-2 gene silencing in the HUVEC cell line, and the effects of COX-2 gene silencing on cell migration in these cells were examined. As a result, the transfection efficiency of the P?AE-based transfection agent for the HUVEC cell line was determined as 90-95%, and the CRISPR efficiency was calculated as 57.12% based on the Western blot results. It was revealed that the P?AE-based nanoparticles used in this study can be used as a good transfection agent in CRISPR/Cas-mediated genome editing in the HUVEC cell line, especially in plasmid transfection.
dc.identifier.doi10.35414/akufemubid.1584459
dc.identifier.endpage719
dc.identifier.issn2149-3367
dc.identifier.issue4
dc.identifier.startpage712
dc.identifier.trdizinid1332085
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1332085
dc.identifier.urihttps://doi.org/10.35414/akufemubid.1584459
dc.identifier.urihttps://hdl.handle.net/20.500.12868/3806
dc.identifier.volume25
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAfyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260121
dc.subjectCRISPR
dc.subjectTransfection
dc.subjectGenom Editing
dc.subjectHUVEC Cell Line
dc.titleInvestigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line
dc.typeArticle

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