Investigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line
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Tarih
2025
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Genome editing technology is a promising, popular technology for diagnosing and treating many diseases. One of the most popular techniques utilized in genome editing research nowadays is the CRISPR/Cas system. The CRISPR/Cas system holds considerable potential for diagnostic and therapeutic applications due to its ease of use, speed, and affordability. The safe and effective delivery of the CRISPR-based genome editing system to the intended human body cells is one of the system's problems. This study investigated the silencing of the COX-2 gene via the CRISPR/Cas system using a P?AE-based transfection agent in the HUVEC cell line. Western blot analysis was performed to confirm COX-2 gene silencing in the HUVEC cell line, and the effects of COX-2 gene silencing on cell migration in these cells were examined. As a result, the transfection efficiency of the P?AE-based transfection agent for the HUVEC cell line was determined as 90-95%, and the CRISPR efficiency was calculated as 57.12% based on the Western blot results. It was revealed that the P?AE-based nanoparticles used in this study can be used as a good transfection agent in CRISPR/Cas-mediated genome editing in the HUVEC cell line, especially in plasmid transfection.
Açıklama
Anahtar Kelimeler
CRISPR, Transfection, Genom Editing, HUVEC Cell Line
Kaynak
Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
4
