Determination of early spectral changes in melanoma cells during epoxomicin-induced apoptotic process

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dc.contributor.authorKucuksayan, Ertan
dc.contributor.authorSircan-Küçüksayan, Aslinur
dc.date.accessioned2026-01-24T12:20:41Z
dc.date.available2026-01-24T12:20:41Z
dc.date.issued2021
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractObjective: Determining the changes in the apoptosis process in cells can provide important information for new treatment and drug research. Apoptotic cells undergo a series of subcellular changes that lead to cell shrinkage and fragmentation. Determining these early changes in the apoptotic process depending on time may provide a new perspective to cell culture studies. The aim of this study is to develop a method in which early spectral changes occurring in the apoptosis process of melanoma cells can be determined depending on time. Methods: In this study, epoxomicin (Epo) was used to induce apoptosis in A375 melanoma cell line and apoptotic dose was determined by MTT method. In order to evaluate the early apoptotic process in a time-dependent manner, measurements were made at five different time points (0.5-6 hours) with a fixed Epo dose. DCFH-DA method was used to measure ROS, which is the most important stimulus of apoptosis. Bax amount was determined by Western Blot technique. Spectroscopic measurements were made with a back-reflection spectroscopy experiment setup consisting of spectrometer, tungsten-halogen light source and fiber optic probe. Apoptosis index values were determined from spectra. Results: Early spectral changes were determined with the spectra measured in the apoptosis time of melanoma cells. A significant difference was found in ROS measurements at 2, 4 and 6 hours compared to control. Cell viability was found to be 70% lower than control at 75 and 100 nM Epo doses after 24 hours. Time-dependent Bax levels were found to increase in all Epo groups as an indicator of apoptosis. Spectroscopic apoptosis index value was found to be compatible with ROS and Bax results at all groups. Conclusion: A new approach has been presented in which spectral changes occurring in the early stage of the apoptosis process in cell culture can be determined by back reflection spectroscopy. This approach has the potential to be developed in cell culture studies as a method that can monitor apoptosis over time without interfering with cell culture conditions. © 2021. All Rights Reserved.
dc.identifier.doi10.5505/TURKHIJYEN.2021.63496
dc.identifier.endpage286
dc.identifier.issn0377-9777
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85117368058
dc.identifier.scopusqualityQ4
dc.identifier.startpage273
dc.identifier.urihttps://doi.org/10.5505/TURKHIJYEN.2021.63496
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4480
dc.identifier.volume78
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20260121
dc.subjectapoptosis
dc.subjectapoptozis
dc.subjectBack reflection spectroscopy
dc.subjectepoksomisin
dc.subjectepoxomicin
dc.subjectGeri yansıma spektroskopisi
dc.subjectmelanoma
dc.subjectmelanoma
dc.titleDetermination of early spectral changes in melanoma cells during epoxomicin-induced apoptotic process
dc.title.alternativeMelanoma hücrelerinde epoksomisinin indüklediği apoptozis sürecinde gerçekleşen erken spektral değişikliklerin belirlenmesi
dc.typeArticle

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