Selenium enhances TRPA1 channel-mediated activity of temozolomide in SH-SY5Y neuroblastoma cells
| dc.contributor.author | Özkal, Birol | |
| dc.contributor.author | Övey, İshak Suat | |
| dc.date.accessioned | 2023-11-30T06:54:50Z | |
| dc.date.available | 2023-11-30T06:54:50Z | |
| dc.date.issued | 2020 | |
| dc.department | ALKÜ, Fakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü | |
| dc.description.abstract | Purpose Neuroblastoma is a malignant solid tumor that originates from the sympathetic nervous system in early childhood. Temozolomide is used for treatment in high-risk groups with low treatment response of neuroblastomas. TRPA1 channels in neuroblastoma cells are calcium permeable channels that can be activated by reactive oxygen species (ROT). In this study, we aimed to evaluate the level of activity of temozolomide and selenium in neuroblastoma cells via TRPA1 channels. Method Seven main groups were formed using SH-SY5Y neuroblastoma cells. The control was divided into temozolomide (TMZ) (100 ?M, 24 h), TMZ+SEL+AP18, SEL (sodium selenite, 100 ?M, 24 h), and SEL+AP18 groups. Intergroup calcium signaling, intracellular reactive oxygen species, caspase-3 and caspase-9, and mitochondrial depolarization analyses were performed by channel activation with TRPA1 agonist cinnamaldehyde in all groups. Results Cytosolic calcium concentration, apoptosis, caspase-3 and caspase-9 activation, mitochondrial membrane depolarization, and ROT levels were higher in TMZ (p < 0.001), TMZ+SEL (p < 0.001), and SEL (p < 0.05) groups than the control group. TRPA1 was lower in TTMZ+AP18, TMZ+SEL+AP18, and SEL+AP18 groups with channel blockers than respectively TMZ, TMZ+SEL, and SEL groups without channel blockers (p < 0.05). Conclusion The use of selenium with temozolomide increased the apoptotic efficacy of temozolomide via TRPA1 channels on tumor cells | |
| dc.identifier.doi | 10.1007/s00381-020-04586-7 | |
| dc.identifier.issue | 6 | en_US |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.uri | https://www.scopus.com/record/display.uri?eid=2-s2.0-85082615949&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=20c4fe371b9b908d4f7e419f791331ff&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=72&s=AF-ID%28%22Alanya+Alaaddin+Keykubat+University%22+60198720%29+AND+SUBJAREA%28MEDI%29&relpos=154&citeCnt=1&searchTerm= | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12868/2446 | |
| dc.identifier.volume | 36 | en_US |
| dc.indekslendigikaynak | Scopus | |
| dc.language.iso | en | |
| dc.relation.ispartof | Child's Nervous System | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | Temozolomide | |
| dc.subject | Neuroblastoma | |
| dc.subject | Oxidative stress | |
| dc.subject | TRPA1 | |
| dc.subject | SH-SY5Y | |
| dc.subject | Selenium | |
| dc.title | Selenium enhances TRPA1 channel-mediated activity of temozolomide in SH-SY5Y neuroblastoma cells | |
| dc.type | Article |
